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This Volume presents generic protocols for wet experimental and computer-based systems and synthetic biology approaches relevant to the field of hydrocarbon and lipid microbiology.
Introduction.- Protocol for the standardisation of transcriptional measurements.- Uracil-excision for assembly of complex pathways.- Quantitative physiology approaches to understand and optimize reducing power availability in environmental bacteria.- Design of orthogonal pairs for protein translation: selection systems for genetically encoding noncanonical amino acids in E. coli.- Phenome-ing microbes.- Systems biology tools for methylotrophs.- Protocols for probing genome architecture of regulatory networks in hydrocarbon and lipid microorganisms.- A practical protocol for genome-scale metabolic reconstruction.- Computer-guided metabolic engineering.- Improving biocontainment with synthetic biology: beyond physical containment.