Hydrocarbon and Lipid Microbiology Protocols

Ultrastructure and Imaging

Häftad, Engelska, 2018

AvTerry J. McGenity,Kenneth N. Timmis,Balbina Nogales

1 409 kr

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This Volume presents key microscopy and imaging methods for revealing the structure and ultrastructure of environmental and experimental samples, of microbial communities and cultures, and of individual cells.

Produktinformation

Utgivningsdatum2018-04-22
Mått178 x 254 x 11 mm
Vikt358 g
FormatHäftad
SpråkEngelska
SerieSpringer Protocols Handbooks
Antal sidor174
FörlagSpringer-Verlag Berlin and Heidelberg GmbH & Co. KG
ISBN9783662569832

Tillhör följande kategorier

Tillverkningsteknik inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik
Patologi inom Medicin
Biokemisk teknik inom Naturvetenskap och teknik

Introduction.- Electron microscopy protocols for the study of hydrocarbon-producing and -decomposing microbes: classical and advanced methods.- Protocol for laser scanning microscopy of microorganisms on hydrocarbons.- Fluorescence microscopy for microbiology.- Imaging bacterial cells and biofilms adhering to hydrophobic organic compounds-water interfaces.- Bacteria-mineral colloid interactions in biofilms - an ultrastructural and microanalytical approach.- Identification of microorganisms in hydrocarbon contaminated aquifer samples by fluorescence in-situ hybridization (CARD-FISH).- Studies of the ecophysiology of single cells in microbial communities by (quantitative) microautoradiography and fluorescence in situ hybridization (MAR-FISH).- Protocol for in situ detection of functional genes of microorganisms by Two-pass TSA-FISH.- Three-dimensional visualization and quantification of lipids in microalgae using confocal laser scanning microscopy.- A Correlative Light-Electron Microscopy (CLEM) protocol for the identification of bacteria in animal tissue, exemplified by methanotrophic symbionts of deep-sea mussels.