Human Airway Inflammation

• Airway Inflammation and Remodeling in Asthma.- Airway Inflammation and Remodeling in Asthma.- Sample Collection.- Biopsy Techniques.- Bronchoalveolar Lavage (BAL).- Nasal Secretions and Exudations.- Sample Collection.- Induced Sputum.- Induced Sputum.- Measurement of Exhaled Nitric Oxide and Carbon Monoxide.- Measurement of Exhaled Hydrocarbons.- Breath Condensate as a Vehicle for Collection of Inflammatory Mediators, Especially Hydrogen Peroxide.- Cell Isolation and Culture.- Airway Epithelial Cells (Primaries vs Cell Lines).- Isolation and Culture of Human Alveolar Type II Pneumocytes.- Pulmonary Artery Endothelial Cells.- Isolation and Culture of Human Airway Smooth Muscle Cells.- Isolation and Purification of Human Mast Cells and Basophils.- Neutrophils.- Macrophages.- Collection, Separation, and Activation of Human T Lymphocytes.- Eosinophils.- Gene Expression/Regulation.- Analysis of Gene Expression.- Analysis of Gene Expression.- The Technique of In Situ Hybridization.- Measurement of Airway Mucin Gene Expression.- Transcription Factors and Inflammatory Gene Regulation.- Mediators.- Inflammatory Mediators in Spontaneously Produced Sputum.- Flow Cytometry.- Measurement of Granulocyte Pharmacodynamics in Whole Blood by Flow Cytometry.- Current In Vitro Models of Leukocyte Migration.- Tracing Intracellular Mediator Storage and Mobilization in Eosinophils.- Measurement of Metalloproteinases.- Immunocytochemistry and Immunohistochemistry.- The Measurement of Cysteinyl Leukotrienes in Urine.- Quantitative Analysis of Cyclooxygenase Metabolites of Arachidonic Acid.- Quantitative Analysis of F2 Isoprostanes.

"Comprehensive and highly practical, the methods presented in this volume provide today's basic and clinical researchers all the major techniques for investigating airway inflammation, and powerfully illuminate many novel targets for emerging drugs." - Journal of Investigational Allergology and Clinical Immunology