Del 71 - Methods in Molecular Medicine

Haemophilus influenzae Protocols

Häftad, Engelska, 2010

Av Mark A. Herbert, Derek W. Hood, E. Richard Moxon

1 999 kr

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In HaemophilusiInfluenzae Protocols, leading research scientists and infectious disease specialists detail in a readily reproducible format the major molecular and immunological techniques for exploring the pathogenicity of H. influenzae. Described with step-by-instructions to ensure robust and successful experimental results, the techniques cover plasmid analysis, proteomics, genomics, DNA array technology, gene expression, mutagenesis (transposon and nontransposon), and structural analysis. These methods illuminate how the bacterium causes disease, as well as how best to develop novel vaccines and antibiotics against the organism.

Produktinformation

Utgivningsdatum2010-11-09
Mått155 x 235 x undefined mm
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Medicine
Antal sidor333
FörlagHumana Press Inc.
ISBN9781617372551

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Sjukdomar och rubbningar inom Medicin

The Pathogenesis of Disease Due to Nontypeable Haemophilus influenzae.- The Pathogenesis of Disease Due to Type b Haemophilus influenzae.- General Methods for Culturing Haemophilus influenzae.- Transformation of Haemophilus influenzae.- Diagnosis of Infection.- Characterization of Plasmids.- Protein Characterization by Two-Dimensional Gel Electrophoresis.- Monitoring Gene Expression Using DNA Arrays.- Gene Expression Technology.- The Genome Sequence of Haemophilus influenzae.- Structural Profiling of Short-Chain Lipopolysaccharides from Haemophilus influenzae.- Mutagenesis of H. influenzae.- Transposon Tn 10.- In Vivo Expression of Bacterial Genes During Human Infections.- ELISA.- Opsonophagocytosis Assay Using Flow-Cytometry.- In Vitro Models of Infection I—Human Respiratory Tissue Organ Culture.- In Vitro Models of Infection II—Human Umbilical Vein Endothelial Cells (HUVECs) System.- Animal Models.

"This excellent book is recommended for investigators involved in laboratory research on H. influenzae from the beginning student to the experienced investigator. The book contains abundant, detailed protocols and practical tips that will be useful for all investigators." - Clinical Infectious Diseases"...an excellent source book and an inspiring reading." - International Journal of Medical Microbiology "In addition to detailed information provided in the individual chapters, the book is also valuable by providing an extensive list of 841 references....there are not many microbiological books of such high quality. The authors present highly specialized information in a comprehensible and friendly way." -Folia Microbioligica