G Protein-Coupled Receptors Part A

Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.

• 1. Generating recombinant peptide ligands for GPCRs and their single step purification2. Ca2+ release assay to study downstream pathways of GPCR signal transduction3. Expression and purification of GPCR interacting protein, arrestin4. Isolation and characterization of chemosensory GPCRs from exosomes5. Characterization of chemosensory bitter taste receptor signalling6. Reporter gene assays for investigating GPCR signaling7. Measuring binding affinity of regulatory motifs (peptides) to purified G proteins8. Measuring nucleotide exchange on purified G proteins9. Qualitative and quantitative measurement of GPCR-G protein activity in living cells10. Computational approaches to study GPCR activation11. Visualization and quantification of GPCR trafficking in mammalian cells by confocal microscopy12. Computational studies of βarrestin activation13. Measuring G protein coupling using Glosensor assay14. Computational investigations into GPCR dimerization15. cAMP Assays in GPCR Drug Discovery16. Assays of adrenal GPCR signaling and regulation: Measuring adrenal beta-arrestin activity in vivo through plasma membrane recruitment