Expansion Microscopy for Cell Biology

Paul Guichard is an expert on the structure of the centriole. From 2007 to 2010, he did his PhD at the Institut Curie (France) under the co-direction of Drs. Tassin and Marco, on the study of the human procentriole assembly using cryo-tomography. From 2011 to 2015, he did his post-doctoral work in the laboratory of Prof. Gönczy at EPFL (Lausanne, Switzerland) where he studied the structure of the centriolar cartwheel by combining cryo-tomography, in vitro assemblies and super-resolution microscopy. In 2015, together with Dr. Hamel, they created the Centriole Architecture Laboratory at the University of Geneva (Switzerland), aiming at revealing the molecular architecture and function of the centriole. Virginie Hamel (previously Hachet) is an expert in Cell Biology and Biochemistry, mainly focusing on understanding the function of the centriole and centrosome. She completed her PhD under the supervision of Iain Mattaj at EMBL (Heidelberg, Germany, 2004) working on the role of importin  in nuclear envelope re-assembly in vitro using Xenopus laevis egg extracts. She then carried out postdoctoral studies in the laboratory of Prof. Gönczy at ISREC/EPFL (Lausanne, Switzerland) from 2005 to 2012, where she became interested in the mechanisms dictating timing of mitotic entry in C. elegans embryos. She next was appointed scientist collaborator in that lab (2013-2015) and started to work on centriole biology. She teamed up with Paul Guichard to specifically dissect the mechanisms of centriole assembly. Since 2015, she is scientific collaborator co-heading the Centriole architecture lab with Prof Paul Guichard in the Cell Biology Department at the University of Geneva (Switzerland). Their lab focuses on deciphering the structural mechanisms governing centriole assembly. To this end, they are combining the use of cell biology methods, in vitro reconstitution assays, cryo-microscopy/cryo-tomography and super-resolution microscopy including expansion microscopy to gain decisive insights into these mechanisms.

• 1. Protein-retention Expansion Microscopy: Improved Sub-cellular Imaging Resolution through Physical Specimen ExpansionPaul Tilberg2. Ultrastructure Expansion Microscopy (U-ExM)Davide Gambarotto, Virginie Hamel and Paul Guichard3. Expansion STED microscopy (ExSTED)Helge Ewers4. Simple multi-color super-resolution by X10 microscopySven Truckenbrodt5. Expansion microscopy imaging of various neuronal structuresJae-Byum Chang6. Mapping the neuronal cytoskeleton using expansion microscopyLukas Kapitein7. Mechanical expansion microscopyBo Wang8. Enhanced expansion microscopy to measure nanoscale structural and biochemical remodelling in single cellsIzzy Jayasinghe9. Application of Expansion Microscopy on Developing Arabidopsis SeedsMichael D. Nodine10. A protocol to expand plant nucleiVeit Schubert11. Expansion microscopy of the mitotic spindleIvana Ponjavić12. Expansion microscopy at the nanoscale. The nuclear pore complex as fiducial landmarkPaolo Bianchini13. Post-labeling expansion microscopy, a promise to go beyond super-resolution limitationsPaul Guichard and Virginie Hamel14. Ex-dSTORM and automated quantitative image analysis of expanded filamentous structuresMarkus Sauer15. Expansion Microscopy on Drosophila Spermatocyte CentriolesAlan Wainman