Enzymology at the Membrane Interface: Interfacial Enzymology and Protein-Membrane Binding

Michael H. Gelb studied chemistry and biochemistry as an undergraduate at the University of California at Davis. His Ph.D. studies with Stephen G. Sligar at Yale University led to a better understanding of the catalytic mechanism of cytochrome P450. As an American Cancer Society Postdoctoral Fellow in the laboratory of the late Robert H. Abeles at Brandeis University, Gelb studied a variety of mechanism-based inactivators of serine proteases and developed fluorinated ketones as tight-binding inhibitors of several classes of proteases. In 1985 Gelb became a faculty member in the Departments of Chemistry and Biochemistry at the University of Washington. Major breakthroughs in the group include the development of methods to properly analyze the action of enzymes on membrane surfaces, the discovery of protein prenylation (farnesylation and geranylgeranylation) in mammalian cells (together with John A. Glomset), the development of Isotope-Coded Affinity Tags (ICAT reagents) for proteomic applications (together with Ruedi Aebersold), and the development of newborn screening for lysosomal storage diseases by mass spectrometry.

• 1. A High-Throughput Flurometric Assay for Lipid-Protein BindingWonhwa Cho2. Fluorescence-based in situ quantitative imaging for cellular lipidsWonhwa Cho3. Recombinant Expression of human and mouse groups I, II, III, V, V and XII Secreted Phospholipases A2Michael H. Gelb and Gerard Lambeau 4. Vesicle-based assay of secreted phospholipase A2 enzymatic activity for high throughput screening of compound librariesMichael H. Gelb5. Cellular Assays for Evaluating Calcium-Dependent Translocation of cPLA2a to MembraneChristina C. Leslie6. Secreted Phospholipase A2 Specificity on Natural Membrane PhospholipidsMakoto Murakami7. Analyses of Calcium-Independent Phospholipase A2beta (iPLA2β) in Biological SystemsSasanka Ramanadham8. Using hydrogen deuterium exchange mass spectrometry to examine protein membrane interactionsJohn E. Burke9. Lipid-binding C2 domains mediate a diverse class of enzymatic functions"Robert V. Stahelin10. Interfacial Enzymes: Membrane Binding, Orientation, Membrane Insertion, and ActivitySuren Tatulian11. Enzymology at the Membrane InterfaceDan M. Raben12. Studying gastric lipase adsorption onto phospholipid monolayers by surface tensiometry, ellipsometry and atomic force microscopyFrédéric Carrière13. Probing conformational changes and interfacial recognition site of lipases with surfactants and inhibitorsFrédéric Carrière14. Measuring Phospholipase D enzymatic activity through biochemical and imaging methodsMichael A. Frohman15. Pld Rotein-Protein Interaction with Intracellular Signaling Molecules, Modulation by Mitogen Pa and Pld’s Gef ActivityJulian G. Cambronero16. Phosphatidic Acid Regulation of PIPKIGuangwei Du