Enzyme Engineering and Evolution: General Methods

Dan Tawfik is Principal Investigator at The Department of Biomolecular Sciences, Weizmann Institute of Science, Israel.

• 1. In vivo site-directed recombination (SDR): An efficient tool to reveal beneficial epistasisJavier Viña-Gonzalez and Miguel Alcalde2. A beginner's guide to molecular dynamics simulations and the identification of cross-correlation networks for enzyme engineeringHaoran Yu and Paul A. Dalby3. Exploration of enzyme diversity: High-throughput techniques for protein production and microscale biochemical characterizationMichal Vasina, Pavel Vanacek, Jiri Damborsky and Zbynek Prokop4. Emulsion-based directed evolution of enzymes and proteins in yeastElizabeth C. Gardner, Ella J. Watkins, Jimmy Gollihar and Andrew D. Ellington5. Remodeling enzyme active sites by stepwise loop insertionMd Anarul Hoque, Yong Zhang, Li Zhi, Li Cui and Yan Feng6. Consensus Finder web tool to predict stabilizing substitutions in proteinsBryan J. Jones, Chi Nok Enoch Kan, Christine Luo and Romas J. Kazlauskas7. The use of consensus sequence information to engineer stability and activity in proteinsMatt Sternke, Katherine W. Tripp and Doug Barrick8. Structure-guided rational design of the substrate specificity and catalytic activity of an enzymeJung Min Choi and Hak-Sung Kim9. Biological fitness landscapes by deep mutational scanningJacob D. Mehlhoff and Marc Ostermeier10. Focused rational iterative site-specific mutagenesis (FRISM)Danyang Li, Qi Wu and Manfred T. Reetz11. Statistical analysis of mutational epistasis to reveal intramolecular interaction networks in proteinsCharlotte M. Miton, John Z. Chen, Kalum Ost, Dave W. Anderson and Nobuhiko Tokuriki12. Machine learning-assisted enzyme engineeringNiklas E. Siedhoff, Ulrich Schwaneberg and Mehdi D. Davari13. Ultrahigh throughput screening for enzyme function in dropletsStefanie Neun, Paul J. Zurek, Tomasz S. Kaminski and Florian Hollfelder