Digital PCR

• Entering the Pantheon of 21st Century Molecular Biology Tools: A Perspective on Digital PCR.- Basic Concepts and Validation of Digital PCR Measurements.- Fundamentals of Counting Statistics in Digital PCR: I Just Measured Two Target Copies, What Does it Mean?.- Control Materials and Digital PCR Methods for Evaluation of Circulating Cell Free DNA Extractions from Plasma.- Multiplex Droplet Digital PCR Protocols for Quantification of GM Maize Events.- Using Droplet Digital PCR to Detect Coinfection of Human Herpesviruses 6A and 6B (HHV-6A and HHV-6B) in Clinical Samples.- Biomarkers in Cerebrospinal Fluid: Analysis of Cell-Free Circulating Mitochondrial DNA by Digital PCR.- Testing of General and Human-Associated Fecal Contamination in Waters.- Analyzing Copy Number Variation with Droplet Digital PCR.- Assessing HER2 Amplification in Plasma cfDNA.- Detection and Quantification of Mosaic Genomic DNA Variation in Primary Somatic Tissues using ddPCR: analysis of Mosaic Transposable-Element Insertions, Copy-Number Variants, and Single-Nucleotide Variants.- Monitoring of Response and Resistance in Plasma of EGFR-Mutant Lung Cancer using Droplet Digital PCR.- Detection of Cancer DNA in Early Stage and Metastatic Breast Cancer Patients.- Droplet Digitial PCR for Miniaml Residual Disease Detection in Mature Lymphoproliferative Disorders.- Quantitation of JAK2 V617F Allele Burden by using the QuantStudio™ 3D Digitial PCR System.- Novel Multiplexing Strategies for Quantification of Rare Alleles using ddPCR.- Identification and Use of Personalized Genomic Markers for Monitoring Circulating Tumor DNA.- Single Color Multiplexed ddPCR Copy Number Measurements and Single Nucleotide Variant Genotyping.- A Universal Droplet Digital PCR Approach for Monitoring of Graft Health after Transplantation using a Pre-Selected SNP Set.- Detection and Quantification of HDR and NHEJ Induced by Genome Editing at Endogenous Gene Loci using Droplet Digital PCR.- DNA Methylation Analysis Using Droplet Digital PCR.- Simultaneous Quantification of Multiple Alternatively Spliced mRNA Transcripts using Droplet Digital PCR.- Using Droplet Digital PCR to Analyze Allele-Specific RNA Expression.- Very Low Abundance Single-Cell Transcript Quantification with 5-Plex ddPCR™ Assays.- Quantification of Circulating MicroRNAs by Droplet Digital PCR using EvaGreen Probeless Chemistry.- Droplet Digital PCR for Absolute Quantification of Extracellular MicroRNAs in Plasma and Serum: Quantification of the Cancer Biomarker has-miR-141.- Droplet Digital™ PCR Next-Generation Sequencing Library QC Assay.- Phasing DNA Markers using Digital PCR.- ddTRAP: A Method for Sensitive and Precise Quantification of Telomerase Activity.- Highly Efficient and Reliable DNA Aptamer Selection Using the Partitioning Capabilities of ddPCR: The Hi-Fi SELEX Method.

“The editors of the volume have succeeded in providing specialists in different sectors with a wide range of technology applications that can be adapted to other platforms or can provide the basis for creating new ones.” (Paolo Catarsi, European Journal of Histochemistry, Vol. 63, 2019)