Del 484 - Methods in Enzymology

Constitutive Activity in Receptors and Other Proteins, Part A

Inbunden, Engelska, 2010

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This volume of Methods in Enzymology covers the current methodology for the detection and assessment of constitutively active proteins. The chapters written by expert authors who are leaders in the field, provide hints and tricks not available in primary research publications.It is extensively referenced, with useful figures and tables throughout the volume.

Expert authors who are leaders in the field
Extensively referenced and useful figures and tables
Provides hints and tricks to facilitate reproduction of methods

Produktinformation

Utgivningsdatum2010-11-26
Mått152 x 229 x undefined mm
Vikt1 220 g
FormatInbunden
SpråkEngelska
SerieMethods in Enzymology
Antal sidor804
FörlagElsevier Science
MedarbetareSimon,MelvinI.
ISBN9780123812988

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Section I-- Identification and Measurement of Constitutive Activity Constitutive activity at the cannabinoid CB1 receptor and behavioral responses Katherine E. Hanlon and Todd W. VanderahDetecting constitutive activity and protean agonism at the cannabinoid-2 receptor. Massimiliano Beltramo, Rossella Brusa, Isabella Mancini, Paola Scandroglio, Massimiliano. BeltramoModulation of constitutive activity of the ghrelin receptor by use of pharmacological tools as well as mutagenesis Birgitte Holst and Jacek Mokrosiński Assessment of constitutive activity and internalization of GPR54 (Kiss1-r). Macarena Pampillo and Andy Videsh BabwahAssessment of Constitutive Activity in EP Prostanoid Receptors Hiromichi Fujino, Toshihiko Murayama, and John W. Reganα1D-Adrenergic receptors: constitutive activity and reduced expression at the plasma membraneJ Adolfo Garcia-Sáinz, M. Teresa Romero-Ávila and Luz del Carmen MedinaConstitutive activity of the histamine H1 receptorSaskia Nijmeijer, Rob Leurs and Henry F VischerConstitutive activity of somatostatin receptor subtypesAnat Ben-Shlomo, Kolja Wawrowsky and Shlomo MelmedAssessment of homologous internalization of constitutively active N111G mutant of AT1 receptorMohiuddin Ahmed Bhuiyan and Takafumi NagatomoMethods to detect cell surface expression and constitutive activity of GPR6Balakrishna M. Prasad, Bettye Hollins and Nevin A. Lambertβ3-adrenoceptor agonists and (antagonists as) inverse agonists: history, perspective, constitutive activity and stereospecific binding. Maria Grazia Perrone and Antonio ScilimatiConstitutive Activity of the Lutropin Receptor and its Allosteric Modulation by Receptor HeterodimerizationDeborah L. SegaloffAssessing Constitutive Activity of Extracellular Calcium-Sensing Receptors in Vitro and in BoneWenhan Chang, Melita Dvorak, and Dolores ShobackConstitutive Activity of Neural Melanocortin Receptors. Ya-Xiong Tao, Hui Huang, Zhi-Qiang Wang, Fan Yang, Jessica N. Williams, and Gregory V. NikiforovichMeasurement of Constitutive Activity of BMP Type I receptors Peter ten DijkeProbing the CA among dopamine D1 and D5 receptors and their mutantsBianca Plouffe, Jean-Philippe D’Aoust, Vincent Laquerre, Binhui Liang and Mario TiberiIdentification of Gain-of-Function Variants of the Human Prolactin ReceptorVincent Goffin, Roman L. Bogorad, and Philippe TouraineInvestigations of Activated ACVR1/ALK2, a Bone Morphogenetic Protein (BMP) Type I Receptor that Causes Fibrodysplasia Ossificans Progressiva Frederick. Kaplan, Petra Seemann, Julia Haupt, Meiqi Xu, Vitali Lounev, Mary Mullins, Eileen M. ShoreIdentification and evaluation of constitutively active thyroid stimulating hormone receptor mutationsJoaquin Lado-Abeal, Leah R. Quisenberry, and Isabel Castro-PiedrasAssessment of constitutive activity of a G protein-coupled receptor, Cpr2, in Cryptococcus neoformans by heterologous and homologous methods Chaoyang Xue, Yina Wang, and Yen-Ping HsuehIn vitro and in vivo assessment of mu opioid receptor constitutive activity. Edward J. Bilsky, Denise Giuvelis, Melissa D. Osborn, Christina M. Dersch, Heng Xu and Richard B. RothmanConstitutively active µ-opioid methodsMark. Connor and John TraynorProtein Kinase Ck2 is a Constitutively-Active Enzyme That Promotes Cell Survival: Strategies To Identify Ck2 Substrates and Manipulate Its Activity In Mammalian Cells Jacob P. Turowec, James S. Duncan, Ashley C. French, Laszlo Gyenis, Nicole A. St. Denis, Greg J. Vilk, and David W. Litchfield Assessment of Ck2 Constitutive Activity In Cancer CellsMaria Ruzzene, Giovanni Di Maira, Kendra Tosoni, and Lorenzo A. PinnaStructural basis of the constitutive activity of protein kinase CK2Birgitte B. Olsen, Barbara Guerra, Karsten Niefind and Olar-Georg Issinger font errorMeasuring the Constitutive Activation of c-Jun N-terminal Kinase IsoformsRyan T. Nitta, Shawn S. Badal, and Albert J. WongMeasurement of constitutive MAPK and PI3K/AKT signaling activity in human cancer cell lines.Keiran S.M. SmalleyConstitutive activity of GPR40/FFA1: Intrinsic or assay dependent?Leigh A Stoddart and Graeme MilliganConstitutive activity of TRP channels: methods for measuring the activity and its outcomeBaruch MinkeMeasurement of Orexin (Hypocretin) and Substance P Effects On Constitutively Active Inward Rectifier K+ Channels in Brain NeuronsYasuko Nakajima and Shigehiro NakajimaCharacterization of G protein receptor kinase 4 and measuring its constitutive activity in vivoBradley T. AndresenVoltage-clamp based methods for the detection of constitutively active acetylcholine-gated IK,ACh channels in the diseased heart Niels Voigt, Samy Makary, Stanley Nattel, Dobromir DobrevAssaying Wave and WASH complex constitutive activities towards the Arp2/3 complex. Emmanuel DERIVERY and Alexis GAUTREAU