Comprehensive Enzyme Kinetics

• Chemical Kinetics.- Kinetics of Monosubstrate Reactions.- Derivation of Rate Equations.- Linear Inhibition.- Hyperbolic and Parabolic Inhibition.- Enzyme Activation.- Kinetics of Rapid Equilibrium Bisubstrate Reactions.- Steady-State Kinetics of Bisubstrate Reactions.- Kinetic Analysis of Bisubstrate Mechanisms.- Substrate Inhibition and Mixed Dead-End and Product Inhibition.- Trisubstrate Mechanisms.- Cooperative and Allosteric Effects.- The pH Dependence of Enzyme Catalysis.- Effects of Temperature on Enzyme Reactions.- Isotope Exchange.- Kinetic Isotope Effects.- Statistical Analysis of Initial Rate and Binding Data.

'As scientific attention turns in the post-genomic era, once again, to the properties of proteins, and particularly to their preeminent role as catalysts, Professor Leskovac's book, Comprehensive Enzyme Kinetics, will have to be on every shelf. It will, however, rarely find itself on the shelf, but much more in the hands of researchers who will daily deploy in the laboratory its wealth of useful information. Prepared under the eye of W.W. Cleland, the demiurge of enzyme kinetics, this magnum opus earns its title of comprehensive. The systematic organization of the volume, which somehow manages completeness in just over 400 pages, leads the reader to a target subject easily. The clarity of exposition will be as welcome to the experienced kineticist as it will be to the beginner. This volume is a singular achievement.' R.L. Schowen, Professor Emeritus Chemistry, Molecular Biosciences and Pharmaceutical Chemistry, University of Kansas 'Comprehensive Enzyme Kinetics is an excellent addition to the enzyme kinetics literature. It touches on all of the topics in the various areas of kinetics that might be of interest to the enzymologist. It will make a fine classroom reference text.' Herbert J. Fromm, Distinguished Professor, Iowa State University