Del i serien Methods in Molecular Biology

Channelrhodopsin

Methods and Protocols

Inbunden, Engelska, 2020

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This book merges approaches in understanding the function of the light-gated ion channels known as channelrhodopsin together with methods addressing how channelrhodopsins can be used to address biomedical questions on a cellular or organismal level.

Produktinformation

Utgivningsdatum2020-09-01
Mått178 x 254 x 29 mm
Vikt983 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor405
FörlagSpringer-Verlag New York Inc.
ISBN9781071608296

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Biologi inom Naturvetenskap och teknik
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Molecular Dynamics Simulations of Channelrhodopsin Chimera, C1C2.- Computing Potential of the Mean Force Profiles for Ion Permeation through Channelrhodopsin Chimera, C1C2.- Nanodisc Reconstitution of Channelrhodopsins Heterologously Expressed in Pichia pastoris for Biophysical Investigations.- Characterizing Channelrhodopsin Channel Properties via Two-Electrode Voltage Clamp and Kinetic Modeling.- Charge Transport by Light-Activated Rhodopsins Determined by Electrophysiological Recordings.- Probing Channelrhodopsin Electrical Activity in Algal Cell Populations.- Two-Photon Optogenetic Stimulation of Drosophila Neurons.- Probing Synaptic Signaling with Optogenetic Stimulation and Genetically-Encoded Calcium Reporters.- Optogenetics to Interrogate Neuron-Glia Interactions in Pups and Adults.- Chronic Optogenetic Pacing of Human Induced Pluripotent Stem Cell-Derived Engineered Cardiac Tissues.- Patterned Optogenetic Stimulation Using a DMD-Projector.- Selecting ChannelrhodopsinConstructs for Optimal Visual Restoration in Differing Light Conditions.- Recording Channelrhodopsin-Evoked Field Potentials and Startle Responses from Larval Zebrafish.- Automated Functional Screening for Modulators of Optogenetically-Activated Neural Responses in Living Organisms.- Optogenetic Interrogation of ChR2-Expressing GABAergic Interneurons after Transplantation into the Mouse Brain.- Application of Targeting-Optimized Chronos for Stimulation of the Auditory Pathway.- Channelrhodopsins for Cell-Type Specific Illumination of Cardiac Electrophysiology.- Optogenetic Control of Cardiac Autonomic Neurons in Transgenic Mice.- Dissecting Mechanisms of Motivation within the Nucleus Accumbens Using Optogenetics.- Optogenetic Stimulation of the Central Amygdala Using Channelrhodopsin.- Optical Manipulation of Perfused Mouse Heart Expressing Channelrhodopsin-2 in Rhythm Control.- Chronic Optogenetic Stimulation in Freely-Moving Rodents.