Cell-Wide Identification of Metabolite-Protein Interactions

Inbunden, Engelska, 2022

Av Aleksandra Skirycz, Marcin Luzarowski, Jennifer C. Ewald

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This thorough volume explores protocols of proteome- and metabolome-wide strategies for the identification of protein-small molecule complexes in different organisms, in order to shed light on these important regulatory interactions.

Produktinformation

Utgivningsdatum2022-10-01
Mått178 x 254 x 21 mm
Vikt714 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor260
FörlagSpringer-Verlag New York Inc.
ISBN9781071626238

Tillhör följande kategorier

Biokemi inom Naturvetenskap och teknik
Cellbiologi inom Naturvetenskap och teknik
Fysiologi inom Medicin

Protein-Metabolite Interactions Shape Cellular Metabolism and Physiology.- Affinity Purification Protocol Starting with a Small Molecule as Bait.- Cellular Thermal Shift Assay for the Detection of Small Molecule-Target Interactions in Arabidopsis Cells.- Solvent-Induced Protein Precipitation for Drug Target Discovery.- Identification of Plant Protein-Metabolite Interactions by Limited Proteolysis-Coupled Mass Spectrometry (LiP-MS).- Limited Proteolysis-Mass Spectrometry to Identify Metabolite-Protein Interactions.- Proteome Integral Solubility Alteration (PISA) for High-Throughput Ligand Target Deconvolution with Increased Statistical Significance and Reduced Sample Amount.- Parallel Analysis of Protein–Protein and Protein–Metabolite Complexes Using a Single-Step Affinity Purification.- Detecting and Characterizing Interactions of Metabolites with Proteins by Saturation Transfer Difference Nuclear Magnetic Resonance (STD-NMR) Spectroscopy.- PROMIS: Co-Fractionation Mass Spectrometry for Analysis of Protein-Metabolite Interactions.- Metabolomics: Going Deeper, Going Broader, Going Further.- Specifics of Metabolite-Protein Interactions and Their Computational Analysis and Prediction.- Biophysical Approaches for the Characterization of Protein-Metabolite Interactions.- Databases and Tools to Investigate Protein-Metabolite Interactions.