Cancer Cell Culture

Methods and Protocols

Inbunden, Engelska, 2023

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This volume explores the latest collection of cell models that are used in preclinical cancer research, and covers both two-dimensional and three-dimensional culturing techniques.

Produktinformation

Utgivningsdatum2023-05-19
Mått178 x 254 x 23 mm
Vikt780 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor290
FörlagSpringer-Verlag New York Inc.
ISBN9781071630556

Tillhör följande kategorier

Onkologi inom Medicin
Medicinsk utrustning och medicinska tekniker inom Medicin
Cellbiologi inom Naturvetenskap och teknik

Cancer Cell Culture: The Basics and Two-Dimensional Cultures.- Cell Cultures at the Air–Liquid Interface and Their Application in Cancer Research.- Three-Dimensional Spheroids for Cancer Research.- Disease Animal Models for Cancer Research.- Generation of Radio-Resistant Prostate Cancer Cells.- Generation and Characterization of an Isogenic Cell Line Model of Radioresistant Oesophageal Adenocarcinoma.- Developing an In Vitro Isogenic Model of Chemotherapy-Resistant Lung Cancer.- Culturing Human Lung Adenocarcinoma Cells in a Serum-Free Environment.- A Method for Culturing 3D Tumoroids of Lung Adenocarcinoma Cells at the Air-Liquid Interface.- Isolation and Cryopreservation of Mononuclear Cells from Peripheral Blood and Bone Marrow of Blood Cancer Patients.- Serum-Free Production of Human Stem Cell-Derived Liver Spheres for Cancer Metastasis Research.- A Self-Assembly Method for Creating Vascularized Tumor Explants Using Biomaterials for 3D Culture.- A Step-by-Step Methodological Guide for Developing Zonal Multicellular Scaffold-Based Pancreatic Cancer Models.- Measuring Immune Cell Movement Towards the Soluble Microenvironment of Human Tissues Using a Boyden Chamber-Based Migration Assay.- A Method for the In Vitro Cytotoxicity Assessment of Anti-Cancer Compounds and Materials Using High Content Screening Analysis.- Testing the Effects of Magnetic Hyperthermia in In Vitro 2D Tumor Cultures.- Cell Viability Assay with 3D Prostate Tumor Spheroids.- Analysis of Cancer Cell Line Secretomes: A Complementary Source of Disease-Specific Protein Biomarkers.