Brain Development

Methods and Protocols

Häftad, Engelska, 2020

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This book provides a thorough introduction to widely used techniques for the study of the intersection between developmental biology and neuroscience, an exceptional area to address and investigate impacting biological questions.

Produktinformation

Utgivningsdatum2020-09-26
Mått178 x 254 x 30 mm
Vikt1 011 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor527
Upplaga2
FörlagHumana Press Inc.
ISBN9781493997343

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Combining BrdU-Labeling to Detection of Neuronal Markers to Monitor Adult Neurogenesis in Hydra.- Reverse Genetic Approaches to Investigate the Neurobiology of the Cnidarian Sea Anemone Nematostella vectensis.- Generating Transgenic Reporter Lines for Studying Nervous System Development in the Cnidarian Nematostella vectensis.- Immunostaining and In Situ Hybridization of the Developing Acoel Nervous System.- Immunostaining of the Embryonic and Larval Drosophila Brain.- Non-Fluorescent RNA In Situ Hybridization Combined with Antibody Staining to Visualize Multiple Gene Expression Patterns in the Embryonic Brain of Drosophila.- Analysis of Complete Neuroblast Cell Lineages in the Drosophila Embryonic Brain via DiI Labeling.- Flybow to Dissect Circuit Assembly in the Drosophila Brain: An Update.- Live Cell Imaging of Neural Stem Cells in the Drosophila Larval Brain.- CRISPR/Cas9 Genome Editing to Study Nervous System Development in Drosophila.- The Red Flour Beetle as Model for Comparative Neural Development: Genome Editing to Mark Neural Cells in Tribolium Brain Development.- A Protocol for Double Fluorescent In Situ Hybridization and Immunohistochemistry for the Study of Embryonic Brain Development in Tribolium castaneum.- Immunohistochemistry and Fluorescent Whole Mount RNA In Situ Hybridization in Larval and Adult Brains of Tribolium.- X-Ray Microscopy of the Larval Crustacean Brain.- Immunolocalization of Neurotransmitters and Neuromodulators in the Developing Crayfish Brain.- Immunostainings in Nervous System Development of the Nematode C. elegans.- Methods in Brain Development of Molluscs.- A Simple Method to Identify Ascidian Brain Lineage Cells at Neural Plate Stages Following In Situ Hybridization.- Spawning Induction and Embryo Micromanipulation Protocols in the Amphioxus Branchiostoma lanceolatum.- In Situ Hybridization and Immunostaining of Xenopus Brain.-Morpholino Studies in Xenopus Brain Development.- Sensitive Multiplexed Fluorescent In Situ Hybridization Using Enhanced Tyramide Signal Amplification and Its Combination with Immunofluorescent Protein Visualization in Zebrafish.- Live Morphometric Classification of Sensory Neurons in Larval Zebrafish.- Immunohistochemistry and In Situ Hybridization in the Developing Chicken Brain.- Gene Silencing in Chicken Brain Development.- Transplantation of Neural Tissue: Quail-Chick Chimeras.- Immunohistochemistry and RNA In Situ Hybridization in Mouse Brain Development.- The Cre/Lox System to Assess the Development of the Mouse Brain.- In Utero Electroporation to Study Mouse Brain Development.