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The use of entomopathogenic nematodes can be included in pest management strategies due to their biocontrol potential. Additionally, bacteria, fungi, and actinomycetes possess biocontrol potential against plant-parasitic nematodes. Beneficial Nematodes and Nematode Antagonistic Bioagents is divided into two sections. The first section (chapters 1-16) covers the isolation, identification, molecular characterization, mass multiplication, formulation, and shelf life of bacteria, fungi, and actinomycetes. The second section (chapters 17-24) focuses on the isolation, identification, mass culturing of insect hosts, mass multiplication, formulation, and field potential of entomopathogenic nematodes against economically significant insect pests.
S. Subramanian, Department of Nematology, Tamil Nadu Agricultural University Coimbatore - 641 003, Tamil Nadu.P. Vetrivelkalai, Department of Nematology, Tamil Nadu Agricultural University Coimbatore - 641 003, Tamil Nadu.M. Sivakumar, Department of Nematology, Tamil Nadu Agricultural University Coimbatore - 641 003, Tamil Nadu.K. Poornima, Department of Nematology, Tamil Nadu Agricultural University Coimbatore - 641 003, Tamil Nadu.
Part-I (Bioagents for Plant Parasitic Nematodes) 1. Biological Control Revisiting the Basic Principles - R.K. Walia 2. Bacterial Antagonists Against Phytonematodes S. Nakkeeran and C. Dheepa 3. Fungal Antagonists Against Phytonematodes A.S. Krishnamoorthy and R. Priyanka 4. Mass Production of Fungal and Bacterial Antagonists S. Nakkeeran, G. Karthikeyan and S. Vinod Kumar 5. Formulation and Registration Protocols for Bioagents S. Nakkeeran, K. Eraivan Arutkani Aiyanathan and M. Muthamilan 6. Bioagents As A Component of IPM S. Harish and S. Rajamanicam 7. Notes for Practical Sessions Preparation of Media for Isolation of Bioagents S. Nakkeeran and C. Dheepa 8. Isolation of Antagonistic Trichoderma, Rhizobacteria and Actinomycetes A.S. Krishnamoorthy and R. Priyanka 9. Mass Multiplication of Trichoderma viride S. Nakkeeran, S. Vasumadhi and S. Vinod Kumar 10. Mass Multiplication of Pseudomonas fluorescens S. Nakkeeran, R. Dheepa and A. Ramanathan 11. Mass Multiplication of Bacillus subtilis S. Harish, S. Rajamanicam and S. Rageshwari 12. Assessing the Shelf life of Bioformulations S. Harish, S. Rajamanicam and S. Rageshwari 13. Extraction of DNA from the Biocontrol Agents L. Rajendran and S. Rageshwari 14. PCR Detection of Antibiotics Producing Genes from Antagonistic Bacillus S. Harish, S. Rajamanicam and S. Rageshwari 15. Agarose Gel Electrophoresis for Detection of Biocontrol Agents S. Nakkeran and S. Rageshwari 16. Isolation of Secondary Metabolites from Culture Filtrate of Trichoderma viride and Chaetomium globosum S. Nakkeran and S. Vinodkumar Part-II (Entomopathogenic Nematodes) 17. Introductory Lecture on Entomopathogenic Nematodes S. Subramanian 18. Mass Production of Corcyra cephalonia Stainton and Greater wax moth, Galleria mellonella S. Sridharan, P.A. Saravanan and T. Manoharan 19. Taxonomy and Identification of Steinernema and Heterorhabditis M. Sivakumar and S. Subramanian 20. Microscopic Examination and Identification of Steinernema and Heterorhabditis K. Poornima 21. Mass Multiplication of Entomopathogenic Nematodes by in vivo and in vitro Methods N. Swarnakumari 22. Basic Laboratory Methods Associated with Entomopathogenic Nematodes P. Vetrivelkalai 23. Field Potential of Entomopathogenic Nematodes Against Different Economically Important Insects C. Sankaranarayanan 24. Field Application of Entomopathogenic Nematodes Formulations and Future Prospects J. Gulsar Banu