Basic Confocal Microscopy

Dr. W. Gray (Jay) Jerome is an Associate Professor of Pathology, Microbiology and Immunology at Vanderbilt University School of Medicine. He has published extensively on microscopy of biological material. His work involves correlating microscopic structural information with biochemical, physiological, and molecular genetic data to provide a comprehensive picture of structure-function relationships. Dr. Jerome is a Fellow of the Microscopy Society of America, a Fellow of the American Association for the Advancement of Science, a Fellow of the American Heart Association and a Past-President of the Microscopy Society of America.  Dr. Robert L. Price is a Research Professor in Cell Biology and Anatomy at the University of South Carolina School of Medicine and Director of the Instrumentation Resource Facility. He has been active in biomedical imaging using confocal microscopy since 1990 and along with Dr. Jerome has taught numerous Confocal Workshops in the United States and Australia.  Dr. Price has received several awards from the University of South Carolina and the Microscopy Society of America, is a Fellow of the Microscopy Society of America, and currently President of the Microscopy Society of America.

• Chapter 1 Introduction and Historical Perspective.- Chapter 2 The Theory of Fluorescence.- Chapter 3 Fluorescence Microscopy.- Chapter 4 Specimen Preparation.- Chapter 5 Labeling Considerations for Confocal Microscopy.- Chapter 6 Digital Imaging.- Chapter 7 Confocal Digital Image Capture.- Chapter 8 Types of Confocal Instruments: Basic Principals and Advantages and Disadvantages.- Chapter 9 Setting the Operating Parameters.- Chapter 10 3D Reconstruction of Confocal Image Data.- Chapter 11 Analysis of Image Similarity and Relationship.- Chapter 12 Ethics and Resources.