Ribosome Biogenesis

Methods and Protocols

Häftad, Engelska, 2022

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Part Five covers modifications that increasethe complexity of rRNAs, and Part Six provides readers with a review of eukaryotic translation and - for the first time - describes a new method to analyze translation in vitro.

Produktinformation

Utgivningsdatum2022-07-08
Mått178 x 254 x 19 mm
Vikt574 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor287
FörlagSpringer-Verlag New York Inc.
ISBN9781071625033

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A Comparative Perspective on Ribosome Biogenesis: Unity and Diversity across the Tree of Life.- Establishment and Maintenance of Open Ribosomal RNA Gene Chromatin States in Eukaryotes.- Analysis of Yeast RNAP I Transcription of Nucleosomal Templates In Vitro.- Specialization of RNA Polymerase I in Comparison to Other Nuclear RNA Polymerases of Saccharomyces cerevisiae.- Structural Studies of Eukaryotic RNA Polymerase I using Cryo-Electron Microscopy.- Preparation of RNA Polymerase Complexes for their Analysis by Single Particle Cryo-Electron Microscopy.- Eukaryotic Ribosome Assembly and Nucleo-Cytoplasmic Transport.- Tethered MNase Structure Probing as Versatile Technique for Analyzing RNPs using Tagging Cassettes for Homologous Recombination in Saccharomyces cerevisiae.- Chemical Modifications of Ribosomal RNA.- In Vitro Selection of Deoxyribozymes for the Detection of RNA Modifications.- Mapping of the Chemical Modificationsof rRNAs.- Non-Radioactive In Vivo Labelling of RNA with 4-thio-uracil.- Translation Phases in Eukaryotes.- Differential Translation Activity using Bio-Orthogonal Non-Canonical Amino Acids Tagging (BONCAT) in Archaea.- Thermofluor-Based Analysis of Protein Integrity and Ligand Interactions.- In Vitro Assembly of a Fully Reconstituted Yeast Translation System for Studies of Initiation and Elongation Phases of Protein Synthesis.