Ion Channels: Channel Biochemistry, Reconstitution, and Function

Daniel L. Minor, Professor, Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA Henry M. Colecraft, John C. Dalton Professor of Physiology and Cellular Biophysics & Cellular Biophysics, Columbia University College of Physicians & Surgeons, New York, NY, USA

• PrefaceDaniel L. Minor Jr. and Henry M. ColecraftStructure/function relationships1. Correlating ion channel structure and functionCrina Nimigean2. TRPV3 Expression and Purification for Structure Determination by Cryo-EMAlexander Sobolevsky3. Methods to study phosphoinositide regulation of ion channelsTibor Rohacs4. Recombinant Expression and Purification of Pentameric Ligand-Gated Ion Channels for Cryo-EM Structural Studies Sudha Chakarpani5. Detection of ligand binding to purified HCN channels using fluorescence-based size exclusion chromatographyAnna MoroniStructural and chemical methods to assess channel dynamics6. Structural Dynamics of Channels and Transporters by High-Speed Atomic Force Microscopy Simon Scheuring7. Crosslinking for gating motions in GluRsAndrew Plested8. Single molecule FRET methodology for investigating glutamate receptorsVasanthi Jayaraman9. Interrogating permeation and gating of Orai channels using chemical modification of cysteine residuesMurali PrakriyaChannel reconstitution and studies in bilayers10. A quantitative flux assay for the study of Cl- channels and transportersAlessio Accardi11. Ion channels reconstitution in lipid bilayersEleonora Zakharian12. Combining in vitro translation with nanodisc technology and functional reconstitution of channels in planar lipid bilayersGehard ThielMethods to assess oligomerization13. The application of Poisson distribution statistics in ion channel reconstitution to determine oligomeric architectureRandy Stockbridge