Del 653 - Methods in Enzymology

Ion Channels: Channel Production and Optical Methods

Inbunden, Engelska, 2021

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Ion Channels Part B, Volume 652 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including NMDAR, Pannexin, and CALHM, Making NaV1.4 and NaV1.7, TRPVs, Purification native nAChRs, GABAR Radu Aricescu, TRPV5/2, NaV1.5, KATP, TRPA1, TREK-1, SARS-CoV-2 3a ion channel, Ion channel conformational dynamics by encoded unnatural amino acid, Fluorescence lifetime measurement of absolute membrane potential, Fluorescent Toxins as Activity Sensors, FRET Analyses of Ion Channel Protein-Protein Interactions, Control of Ion Channel Gating with Photo-Switchable Tweezers, and Counting Subunits in Kv Channel Complexes.

Provides the authority and expertise of leading contributors from an international board of authors
Presents the latest release in the Methods in Enzymology series

Produktinformation

Utgivningsdatum2021-06-09
Mått152 x 229 x undefined mm
Vikt770 g
FormatInbunden
SpråkEngelska
SerieMethods in Enzymology
Antal sidor420
FörlagElsevier Science
ISBN9780323853767

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PrefaceDaniel L. Minor Jr. and Henry M. ColecraftMethods to produce and purify ion channels1. Effective production of oligomeric membrane proteins by EarlyBac Hiro Furukawa2. Preparation of the Transient Receptor Potential Vanilloid 2 (TRPV2) channel for structural studiesLejla Zubcevic3. Production and Purification of TRPV2 and TRPV5 for Cryo-Electron Microscopy StudiesVera Moiseekova-Bell4. Sample Preparation of the Human TRPA1 Ion Channel for Cryo-EM StudiesSeok-Yong Lee5. Expression and Purification of the Cardiac Sodium Channel NaV1.5 for Cryo-EM Structure DeterminationWilliam A. Catterall6. Structural determination of human Nav1.4 and Nav1.7 using single particle cryo-electron microscopyNieng Yan7. Production and Purification of ATP-Sensitive Potassium Channel Particles for Cryo-Electron MicroscopyShow-Ling Shyng8. Production of K2P2.1 (TREK-1) for structural studiesDaniel L. Minor Jr.9. Purification of a native nicotinic receptorRyan Hibbs10. SARS-CoV-2 3a expression, purification, and reconstitution into lipid nanodiscs Stephen BrohawnOptical methods for probing and controlling channel function11. Studying Ion Channel Conformation Dynamics by Encoding Coumarin as Unnatural Amino AcidSebastian E. Brauchi12. Fluorescence lifetime measurement of absolute membrane potentialEvan Miller13. Fluorescent Toxins as Ion Channel Activity Sensors Jon Sack14. Probing Ion Channel Macromolecular Interactions using Fluorescence Resonance Energy TransferManu Ben-Johny15. Manipulation of Ion Channel Gating with Photoswitchable TweezersThomas Grutter16. Counting Subunits in Kv Channel ComplexesRikard Blunck