Immune Mediators in Cancer

Methods and Protocols

Häftad, Engelska, 2021

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This book provides a comprehensive collection of classic and cutting-edge methodologies as well as bioinformatics and genome-editing approaches that are used to quantify immune mediators and analyze their function and biological activity in cancer cells and tissues.

Produktinformation

Utgivningsdatum2021-01-15
Mått178 x 254 x 21 mm
Vikt728 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor373
FörlagSpringer-Verlag New York Inc.
ISBN9781071602492

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A Useful Guide for Analysis of Immune Mediators in Cancer by Fluorochrome (Luminex) Technique.- Analysis of Circulating HMGB1 in Human Serum.- Analysis of IL-22 and Th22 Cells by Flow Cytometry in Systemic Lupus Erythematosus.- Application of Immunohistochemistry in Basic and Clinical Studies.- Interleukin-1 (IL-1) Immunohistochemistry Assay in Oral Squamous Cell Carcinoma.- Luminex xMAP Assay to Quantify Cytokines in Cancer Patient Serum.- Detection of Cytokine Receptors Using Tyramide Signal Amplification for Immunofluorescence.- Analysis of IFNg-Induced Migration of Ovarian Cancer Cells.- Interleukin-8-Induced Invasion Assay in Triple Negative Breast Cancer Cells.- Interleukin-8 Induces Proliferation of Ovarian Cancer Cells in 3D Spheroids.- Detection of Ferroptosis by BODIPY™ 581/591 C11.- Methods for Studying TNFα-Induced Autophagy.- Isolation of Antibody Binders to MISIIR from a Phage Display Library by Sorting.- Measuring Chimeric Antigen Receptor T Cells (CAR T Cells) Activation by Coupling Intracellular Cytokine Staining with Flow Cytometry.- Analysis of Interleukin-4-Induced Class Switch Recombination in Mouse Myeloma CH12F3-2 Cells.- Single-Stranded DNA Aptamers against TNF and Their Potential Applications.- Probing Metabolic Changes in IFNγ-Treated Ovarian Cancer Cells.- Immunoblotting Analysis of Intracellular PD-L1 Levels in Interferon-g-Treated Ovarian Cancer Cells Stably Transfected with Bcl3 shRNA.- Flow Cytometry Analysis of Surface PD-L1 Expression Induced by IFNg and Romidepsin in Ovarian Cancer Cells.- Analysis of PD-L1 Transcriptional Regulation in Ovarian Cancer Cells by Chromatin Immunoprecipitation.- Real-Time PCR Assay for the Analysis of Alternative Splicing of Immune Mediators in Cancer.- Combined Single-Cell Measurement of Cytokine mRNA and Protein in Immune Cells.- Microscopic Methods for Analysis of Macrophage-Induced Tunneling Nanotubes.- Optogenetics: Rho GTPases Activated by Light in Living Macrophages.- High-Throughput RNA interference Screen Targeting Synthetic-Lethal Gain-of-Function of Oncogenic Mutant TP53 in Triple Negative Breast Cancer.- Discovering Transcription Factor Non-Coding RNA Targets Using ChIP-Seq Analysis.- Phylogenetic Analyses of Chemokine Receptors from Sequence Retrieval to Phylogenetic Trees.- Generation of IL17RB Knockout Cell Lines Using CRISPR/Cas9-Based Genome Editing.- Engineering Mutation Clones in Mammalian Cells with CRISPR/Cas9.