Tissue-Resident Macrophages

Methods and Protocols

Inbunden, Engelska, 2023

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The contents aim to describe the current knowledge about macrophage development and function which forces the scientific field to move beyond the previously described M1/M2 macrophage paradigm to be able to dissect macrophage functions within their specific niches during health and disease.

Produktinformation

Utgivningsdatum2023-08-29
Mått178 x 254 x 38 mm
Vikt1 303 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor576
FörlagSpringer-Verlag New York Inc.
ISBN9781071634363

Tillhör följande kategorier

Onkologi inom Medicin
Biologi inom Naturvetenskap och teknik
Cellbiologi inom Naturvetenskap och teknik

Macrophage Development and Function.- Fate Mapping Macrophages: From Ontogeny to Functions.- Studying Autophagy in Microglia: Overcoming the Obstacles.- Hemocyte Nuclei Isolation from Adult Drosophila melanogaster for snRNA-Seq.- Isolation of Tissue Macrophages in Adult Zebrafish.- Genetic and Immunohistochemistry Tools to Visualize Rat Macrophages In Situ.- Phenotyping of Macrophages in Human Immune System Mice.- Fate-Mapping of Yolk Sac-Derived Macrophages.- Fate-Mapping of Hematopoietic Stem Cell-Derived Macrophages.- Isolation and Flow Cytometry Analysis of Macrophages from White Adipose Tissue.- Isolation and Flow Cytometry Analysis of Macrophages from the Dermis.- Isolation and Flow Cytometry Analysis of Macrophages from the Kidney.- Isolation and Flow Cytometry Analysis of Intestinal Macrophages.- Isolation and Characterization of Testis Macrophages Using Flow Cytometry.- Studying Macrophages in the Murine Fatty Liver Using Flow Cytometry and Confocal Microscopy.- Isolation, Ex Vivo Expansion, and Lentiviral Transduction of Alveolar Macrophages.- Translatome Profiling of Tissue-Resident Macrophages Using the RiboTag Approach.- Spectral Flow Cytometry Analysis of Resident Tissue Macrophages.- Unveiling Macrophage Heterogeneity and Their Spatial Distribution Using Multiplexed Tissue Imaging.- 3D Imaging of Macrophages in Complete Organs.- Whole-Mount Imaging of Adipose Tissue Macrophages.- Functional In Vivo Imaging of Macrophages.- Elucidating Immune Monitoring of Tissue-Resident Macrophages by Intravital Microscopy.- Combined Host-Pathogen Fate Mapping to Investigate Lung Macrophages in Viral Infection.- Measuring the Metabolic State of Tissue-Resident Macrophages via SCENITH.- Analyzing Fcg-Receptor Interactions on Monocytes with the Proximity Ligation Assay (PLA).- Studying Efferocytosis Dynamics in Tissue-Resident Macrophages Ex Vivo.- Monitoring of Inflammasome Activation of Macrophages and Microglia In Vitro, Part 1: Cell Preparationand Inflammasome Stimulation.- Monitoring of Inflammasome Activation of Macrophages and Microglia In Vitro, Part 2: Assessing Inflammasome Activation.- Detection of G-Quadruplex DNA Structures in Macrophages.- Adaptation of Human iPSC-Derived Macrophages Towards an Alveolar Macrophage-Like Phenotype Post Intra-Pulmonary Transfer into Murine Models.- Tackling Tissue Macrophage Heterogeneity by SplitCre Transgenesis.- Automated Cell Counting of Macrophages In Situ.- Morphometric Analyses of Macrophages.- Combined Analysis of mRNA Expression and Open Chromatin in Microglia.