Techniques and Protocols for Evaluating Nanosystems

Dr. Natassa Pippa is an Assistant Professor in the Department of Pharmaceutical Technology at the National and Kapodistrian University of Athens, Greece. Her research focuses on pharmaceutical technology, specifically the design and development of nanoparticles, such as liposomes, micelles, and hydrogels, for drug delivery and targeting. She completed her master's degree in "Industrial Pharmacy" in 2012 and her PhD thesis in 2015 at the Department of Pharmacy, University of Athens.Dr. Pippa has been awarded three scholarships for postdoctoral research in Greece and France. She has collaborated with the pharmaceutical industry in the development of drugs, cosmetics, and nutritional supplements.Dr. Nefeli Lagopati is an Assistant Professor in Biology-Nanomedicine at the Department of Biology in the School of Medicine at the National & Kapodistrian University of Athens (NKUA), Greece. She completed her PhD at the Faculty of Biology in the School of Science, NKUA, in collaboration with the Laboratory for the Research on Cell & Matrix Biochemistry/Pathobiology at the Institute of Biosciences and Applications, and the Laboratory of Nanotechnology processes for solar energy conversion and environmental protection at the Institute of Nanoscience and Nanotechnology, National Center for Scientific Research "Demokritos".Dr. Lagopati's research interests include the multidisciplinary field of nanomedicine in cancer treatment. Her specific focus is on the apoptotic effect induced by oxidative stress and the anticancer activity of nanomaterials. Over the past decade, she has dedicated her work to the development of drug delivery systems, biomaterials, hybrid materials, cellular senescence, oxidative stress molecular mechanisms, radiobiology, Monte Carlo simulation, dosimetry in nuclear medicine, and more recently, projects related to SARS-CoV-2.

• 1. The properties, the classes, and the applications of nanoparticulate systems2. Differential Scanning Calorimetry and micro-Differential Scanning Calorimetry3. Thermogravimetric analysis (TGA)4. Isothermal Titration Calorimetry (ITC)5. Dynamic-Static-Electrophoretic Light Scattering6. Neutron Scattering Techniques7. Transmission Electron Microscopy8. Atomic Force Microscopy9. Confocal Microscopy10. Analytical Techniques (i.e. HPLC, BET, etc.)11. Optical spectroscopic techniques (PL, XRD, XPS, etc.)12. Optical spectroscopic techniques (UV-Vis, FTIR, Raman, etc.)13. Statistical and AI-Based Optimization Techniques (Taguchi Method, Machine Learning, Data Mining, etc.)14. Nanotoxicity studies15. The usage of the techniques of the characterization of nanoparticulate systems in the pharmaceutical industry16. Characterization techniques for the design and the development nanosimilars and “off-patent” nanomedicines17. The requirements for the characterization of the nanoparticulate systems from the regulatory authorities18. Conclusions and future perspectives