Spermatogenesis

Methods and Protocols

Inbunden, Engelska, 2025

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Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Produktinformation

Utgivningsdatum2025-07-03
Mått178 x 254 x 23 mm
Vikt778 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor296
FörlagSpringer-Verlag New York Inc.
ISBN9781071646977

Tillhör följande kategorier

Biovetenskap inom Naturvetenskap och teknik
Cellbiologi inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik

Profiling Histone Modifications in Differentiating Mouse Spermatogonia with CUT&Tag.- Quantitative CUT&Tag for Epigenomic Profiling of Mouse Germ Cells.- Spatial Transcriptomic Analyses of Spermatogenesis.- Identification of RNA Targets of Highly Conserved RNA Binding Proteins Essential for Animal Sperm Development.- Generation of Organotypic Testicular Organoids from Rat and Pig Primary Cells in Microwell Culture.- Inhibiting Aldehyde Dehydrogenase Function Using WIN 18,446 to Synchronize Spermatogenesis.- Individualization of Testicular Cells for Single-Cell Subcellular Immunocytochemistry and Cell Culture Applications.- Isolation of Pig or Rodent Sertoli Cells for Use in Transplantation.- Density Gradient-Based Separation of Testicular Cell Types Using a STA-PUT Apparatus.- Antibody Labeling of Mouse Spermatocyte Chromosome Spreads to Study Meiosis.- Transillumination-Assisted Microdissection for Precise Staging of Seminiferous Tubules in Mice.- Immunofluorescence Staining of the Manchette and Developing Sperm Flagella in Mouse.- Methods of Mammalian In Vitro Sperm Capacitation to Study Sperm Physiology.- Analysis of Motion Characteristics and Plasma Membrane Intactness (Viability) in Sperm from Domestic Animals.- Methods to Determine Oxidative Stress in Spermatozoa.- Methods to Evaluate DNA Damage in Spermatozoa.- Cre Deleter Mouse Lines for Conditional Knockout of Genes in Testis Cell Types.