Screening, Characterization of Xylanase Producing Microorganisms

Xylanase is an enzyme of glycosyl hydrolase family (11) that can easily break the beta-1, 4-d-xylosidic linkage of xylan, which is a major constituent of hemicelluloses. These days, xylanase are the enzymes on demand in pulp and paper industry. But for product enhancement, they need cellulase free xylanase.samples collected from the waste yard of food ndustry.First, =CSPY media was prepared. For enhancing the xylanase producing ability, wood scrapings were used as xylan substrate. From the first stage screening, actinomycete culture is obtained that has shown normal level cellulase free xylanase activity. For higher level activity, other natural substrates like corn cobs, baggaserice bran and pure xylan are used for optimization. This optimization process may enhance the production of cellulase free xylanase that could industrially fruitful in better pulp bleaching. Cellulase assay is done to confirm the absence of cellulase in the strains as cellulase free Xylanase is required. Strain improvement of the selected strains is done with EtBr technique and UV radiation to compare which technique is more fruitful as enhancing the production of required enzyme.