Physical Methods in Plant Sciences

Häftad, Engelska, 2011

Av Hans-Ferdinand Linskens, John F. Jackson

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Latest techniques for the analysis of plant cell or tissue structure and the registration of physiological pathways are topics of this volume. The subjects include: - Laser Doppler Vibrometer Measurements of Leaves; - Laster Physical Methods. Laser Microprobe Mass Spectrometry; - Triplet States in Photosynthesis: Linear Dichroic Optical Difference Spectra via Magnetic Resonance; - Fast Atom Bombardment Mass Spectrometry; -Microdissection and Biochemical Analysis of Plant Tissues; - Photoacoustic Spectroscopy - Photoacoustic and Photothermal Effects; - Membrane Operational Impedance of Spectra of Plant Cell.

Produktinformation

Utgivningsdatum2011-12-05
Mått170 x 242 x 17 mm
Vikt531 g
FormatHäftad
SpråkEngelska
SerieMolecular Methods of Plant Analysis
Antal sidor283
FörlagSpringer-Verlag Berlin and Heidelberg GmbH & Co. KG
ISBN9783642836138

Tillhör följande kategorier

Botanik inom Naturvetenskap och teknik
Växtböcker inom Djur och natur
Biovetenskap inom Naturvetenskap och teknik
Agronomi inom Naturvetenskap och teknik
Biokemi inom Naturvetenskap och teknik
Cellbiologi inom Naturvetenskap och teknik
Skogsbruk inom Naturvetenskap och teknik

Laser—Doppler Vibrometer Measurements of Leaves.- 1 Introduction.- 2 Materials and Methods.- 3 Two Simple Models for Vibrating Leaf Tissue.- 4 Experiments and Results with Clamped Leaves.- 5 Experiments and Results with Free Hanging Leaves.- 6 Discussion.- References.- Triplet States in Photosynthesis: Linear Dichroic Optical Difference Spectra via Magnetic Resonance.- 1 Why Triplet States Are of Interest.- 2 Information Obtainable from ODMR Spectroscopy.- 3 Applications of ODMR in Photosynthesis.- 4 Conclusions and Prospects.- References.- Laser Physical Methods: Laser Microprobe Mass Spectrometry.- 1 Introduction.- 2 Historical Survey.- 3 Instrumentation.- 4 Specimen Preparation.- 5 Features of the Instrument.- 6 Experimental Parameters of Different Groups.- 7 Ion Formation Mechanisms and Characteristics of Spectra.- 8 Efficiency of Different Microprobe Methods.- 9 LAMMA Applications, Inorganic Ions.- 10 LAMMA Applications Organic Compound Analysis.- 11 Stable Isotopes as Markers.- References.- Fast Atom Bombardment Mass Spectrometry.- 1 Introduction to Fast Atom Bombardment Mass Spectrometry.- 2 Quantification and Stable Isotope Analysis of Quaternary Ammonium Compounds.- 3 Quantification and Stable Isotope Analysis of Amino Acids.- 4 Analysis of Oligosaccharides.- 5 Analysis of Polypeptides.- 6 Analysis of Glycoproteins.- 7 Nucleotide Analysis.- 8 Structural Characterization of Miscellaneous Secondary Plant Products.- 9 Concluding Remarks.- 10 References.- Microdissection and Biochemical Analysis of Plant Tissues.- 1 Introduction.- 2 Freeze Stop.- 3 Storage of Frozen Tissues.- 4 Freeze-Drying.- 5 Sample Containers.- 6 Storage of Freeze-Dried Material.- 7 Dissection of Tissue.- 8 Determination of Sample Mass.- 9 Biochemical Analysis of Samples.- 10 Example for theComplete Procedure: Determination of Fumarase Activity.- 11 Examples for Application: Intercellular Compartmentation of Physiological and Biochemical Properties in Plant Tissues.- 12 Final Remarks.- References.- Photoacoustic Spectroscopy — Photoacoustic and Photothermal Effects.- 1 Introduction.- 2 Physics of the Photoacoustic Effect.- 3 Measuring Systems.- 4 Applications in Plant Analysis.- 5 Further Development.- References.- Membrane Operational Impedance Spectra of Plant Cells.- 1 Introduction.- 2 The Laplace Transform.- 3 Measuring Techniques.- 4 Data Analysis Technique.- 5 The Concept of Membrane Capacitance.- 6 Conclusion.- References.- Image Instrumentation Methods of Plant Analysis.- 1 Introduction.- 2 Image Sensor Selection and Processing System.- 3 TV Spectral Image Instrumentation.- 4 Remote-Control Light Microscope System.- 5 Image Instrumentation of Chlorophyll Fluorescence Transients.- 6 Thermal Image Instrumentation.- 7 Computed Tomography.- References.- Energy Dispersive X-Ray Analysis.- 1 Introduction.- 2 Principles — Application and Physics.- 3 The Specimen.- 4 The Spectrum.- 5 Maps and Line Scans.- 6 Quantitative Analysis.- 7 Alternatives to EDX.- 8 Conclusions.- References.