Pharmacological evaluation of acetyl-11-α-ketoβ-boswellic acid mediate

Mastitis is an important economic disease-causing production loss in the dairy industry. Antibiotics are becoming ineffective in controlling mastitis due to the emergence of resistant strains requiring the development of novel therapeutic agents. In this study, the authors present the phytochemical synthesis of silver nanoparticles (AgNPs) with acetyl-11--keto-boswellic acid and evaluation of their activity in Staphylococcus aureus induced murine mastitis. Boswellic acid-mediated AgNP (BANS) were oval, polydispersed (99.8 nm) with a minimum inhibitory concentration of 0.033 g ml1 against S. aureus, inhibitory concentration (IC50) of 30.04 g ml1 on mouse splenocytes and safe at an in vivo acute oral dose of 3.5 mg kg1 in mice. Mastitis was induced in lactating mice by inoculating S. aureus (log10 5.60 cfu) and treated 6 h post-inoculation with BANS (0.12 mg kg1, intramammary and ip), and cefepime (1 mg kg1, ip). BANS treatment significantly (P < 0.05) reduced bacterial load, CRP, SOD, CAT activities and neutrophil infiltration in affected mammary glands. BANS could be a potential therapeutic agent for managing bovine mastitis.