Osteoporosis and Osteoarthritis

Inbunden, Engelska, 2020

AvAndre J. van Wijnen,Marina S. Ganshina,Andre J. Van Wijnen,Andre J. van Wijnen

2 229 kr

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This detailed second edition provides key updates on well-established methods as well as coverage of recently emerging sophisticated techniques and methods that are essential for an in-depth and state-of-the-art understanding of skeletal development and homeostasis and the pathological mechanisms that cause skeletal degeneration.

Produktinformation

Utgivningsdatum2020-09-27
Mått178 x 254 x 23 mm
Vikt794 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor302
Upplaga2
FörlagSpringer-Verlag New York Inc.
ISBN9781071609880

Tillhör följande kategorier

Isolation of Murine and Human Osteocyte.- Expansion and Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells.- A Novel Enzymatic Digestion Approach for Isolation and Culture of Rodent Bone Marrow Mesenchymal Progenitors.- Isolation of Nucleus Pulposus and Annulus Fibrosus Cells from the Intervertebral Disc.- Engineering Cartilage Tissue by Co-Culturing of Chondrocytes and Mesenchymal Stromal Cells.- Generation of Induced Pluripotent Stem Cells.- Specimen Preparation for Single Cell Sequencing Analysis of Skeletal Cells.- Mapping 5-Hydroxymethylcytosine (5hmC) Modifications in Skeletal Tissues Using High-Throughput Sequencing.- Using FRAP to Quantify Changes in Transcription Factor Dynamics after Cell Stimulation: Cell Culture, FRAP, Data Analysis, and Visualization.- Quantitative Molecular Models for Biological Processes: Modeling of Signal Transduction Networks with ANIMO.- Generation and Characterization of Mouse Models for Skeletal Disease.- Drill Hole Models to Investigate Bone Repair.- Generation and Experimental Outcomes of Closed Femoral Fracture in Mice.- Mouse Models of Osteoarthritis: Surgical Model of Post-Traumatic Osteoarthritis Induced by Destabilization of the Medial Meniscus.- Immunostaining of Skeletal Tissues.- Mapping Regional Cortical Bone Responses to Local Changes in Loading and Systemic Stimuli.- Pain and Activity Measurements.