Lysosomes and Lysosomal Diseases

Prof. Frances Platt obtained her PhD from the University of Bath, UK, and was a post-doctoral fellow at Washington University Medical School in St. Louis, USA. She was a Lister Institute Senior Research Fellow and a Reader at the University of Oxford. Prof. Platt’s main research interests include the biology and pathobiology of glycosphingolipids. Her research led to the development of miglustat for the treatment of glycosphingolipid storage diseases. In 1999, Prof. Platt was awarded the Alan Gordon Memorial Award and the Horst Bickel Award for advances in metabolic disease therapy. She was elected a fellow of the Academy of Medical Sciences in 2011. Dr Platt obtained his PhD from the University of Bath, UK and was a post-doctoral fellow at Washington University in St Louis. Since his return to UK he has worked at University of Oxford in the Sir William Dunn School of Pathology and Nuffield Department of Medicine. He has very recently become a senior research fellow in the Department of Pharmacology. His research interests are in innate immunity, particularly the role of specific pattern recognition receptors in immune and inflammatory responses.

• 1. Methods for Monitoring Lysosomal MorphologyBethan S. Kilpatrick, Emily R. Eden, Leanne N. Hockey, Clare E. Futter and Sandip Patel2. A Rapid Method for the Preparation of Ultra-pure, Functional Lysosomes Using Funcionalised Superparamagnetic Iron Oxide NanoparticlesMathew William Walker and Emyr Lloyd-Evans3. TFEB and the CLEAR NetworkCarmine Settembre and Diego L. Medina4. Biosynthesis, Targeting and Processing of Lysosomal Proteins: Pulse-Chase Labeling and Immune PrecipitationSandra Pohl and Andrej Hasilik5. Measuring Lysosomal pH by Fluorescence MicroscopyJohnathan Canton and Sergio Grinstein6. Lysosome Fusion in Cultured Mammalian CellsNicholas A. Bright, Lena Wartosch and J. Paul Luzio7. RNAi Screens of Lysosomal TraffickingSalil Garg, Michael B. Brenner8. Approaches for Plasma Membrane Wounding and Assessment of Lysosome-mediated Repair ResponsesCorrotte, M, Castro-Gomes, T, Koushik, A.B. and Andrews, N.W9. Imaging Approaches to Measuring Lysosomal CalciumAnthony J. Morgan, Lianne C. Davis, Antony Galione10. Lysosome ElectrophysiologyXi Zoë Zhong and Xian-Ping Dong11. Reconstitution of Lysosomal Ion Channels into Artificial MembranesElisa Venturi and Rebecca Sitsapesan12. Fluorescence Methods for Analysis of Interactions Between Ca2+ Signalling, Lysosomes and Endoplasmic ReticulumDavid L. Prole, Cristina I. López-Sanjurjo, Stephen C. Tovey and Colin W. Taylor13. Methods for the Quantification of Lysosomal Membrane Permeabilization: a Hallmark of Lysosomal Cell DeathSonja Aits, Marja Jäättelä and Jesper Nylandsted14. Measuring the Phagocytic Activity of CellsNick Platt and Paul Fineran15. Detection and Quantification of Microbial Manipulation of Phagosomal FunctionMaria Podinovskaia, David G. Russell16. Measuring Relative Lysosomal Volume for Monitoring Lysosomal Storage DiseasesDanielle te Vruchte, Kerri L. Wallom and Frances M. Platt17. Quantifying Storage Material Accumulation in Tissue SectionsJonathan D Cooper, Helen R Brooks, Hemanth R Nelvagal18. Laboratory Diagnosis of Niemann-Pick disease Type C: The Filipin Staining TestMarie T. Vanier and Philippe Latour

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