Heterologous Expression of Membrane Proteins

• Heterologous Expression of Membrane Proteins for Structural Analysis.- Production of Membrane Proteins in Escherichia coli and Lactococcus lactis.- Mammalian Membrane Receptors Expression as Inclusion Bodies in Escherichia coli.- Expression of Membrane Proteins at the Escherichia coli Membrane for Structural Studies.- Membrane Protein Expression in Lactococcus lactis.- Heterologous Expression of Human Membrane Receptors in the Yeast Saccharomyces cerevisiae.- Mammalian Membrane Protein Expression in Baculovirus-Infected Insect Cells.- Expression of Membrane Proteins in Drosophila Melanogaster S2 Cells: Production and Analysis of a EGFP-Fused G Protein-Coupled Receptor as a Model.- Membrane Protein Expression in the Eyes of Transgenic Flies.- Expression of Mammalian Membrane Proteins in Mammalian Cells Using Semliki Forest Virus Vectors.- Membrane Protein Expression in Cell-Free Systems.- Practical Considerations of Membrane Protein Instability during Purification and Crystallisation.- Membrane Protein Solubilization.- Amphipols and Fluorinated Surfactants: Two Alternatives to Detergents for Studying Membrane Proteins In vitro.- Heterologous Expression and Affinity Purification of Eukaryotic Membrane Proteins in View of Functional and Structural Studies: The Example of the Sarcoplasmic Reticulum Ca2+-ATPase.

From the reviews:“Well-illustrated book provides an overview of different heterologous expression systems available to produce membrane proteins for structural analysis to facilitate understanding of how these proteins function and how their functions can be modified by drug treatment. … Written for students and experienced researchers, the book also will be useful for clinicians interested in the care of patients with cancer … . provides valuable information on membrane proteins for novice and experienced researchers. Clinicians will find it extremely useful for developing methods and protocols for research.” (Omer Iqbal, Doody’s Review Service, July, 2010)