Hemoglobins, Part B: Biochemical and Analytical Methods

Hemoglobin has been involved in the most significant advances in our understanding of modern genetics and molecular biology. Now, hemoblobin is again central to a new area: development of artificial blood (blood substitute.) This volume of Methods in Enzymology and its companion, Volume 232, will be indispensable to anyone with a serious interest in this emerging field. They completely update and extend the information presented in Volume 76 published a decade ago.

• Preparation, purification, and analysis of human hemoglobin and of various non-human hemoglobins
• Preparation of hemoglobin hybrids and of model heme compounds
• Preparation of chemically modified hemoglobins and of recombinant hemoglobins
• Analytical procedures presented for studying hemoglobin stability and degradation and for the formation of xenobiotic adducts of hemoglobin