Helicase Enzymes Part A

Michael grew up in a western suburb of Chicago called Aurora, IL, or better known as Wayne’s World. He got his BS in Psychology and Chemistry at the University of Illinois, Urbana-Champaign. After which, he employed as a technician working on analytical method development at Abbott Laboratories. From 1998-2002 he was a PhD student at Penn State University working in Stephen Benkovic lab on the Bacteriophage T4 replisome assembly and dynamics. In 2003, he started a postdoctoral fellowship in Ron Laskey’s laboratory at the University of Cambridge/Hutchison MRC in the UK. In 2006, he started his independent career at the University of Pittsburgh Chemistry department. Starting 2014, he moved his laboratory to Baylor University, where he currently lives with his family. There, the Trakselis laboratory is currently studying aspects of DNA replication and repair spanning from the very in vitro biochemical to the very in vivo cell and human biology.

• 1. Fluorescence loading assay for hexameric helicasesJames Berger2. Use of substrates with multiple fluorophores to simultaneously monitor unwinding of multiple duplexesAlicia Byrd3. In silico reconstitution of MCM helicase loading and activation using time resolved cryo-EMAlessandro Costa4. Helicase uncoupling to study nacent strand decayJames Dewar5. Investigating structure and dynamics of Twinkle helicase with Cryo-EM and high-speed AFMYang Gao6. Measuring helicase contributions to BIRGreg Ira7. Crosslinking to define helicase/DNA interactionsJames L. Keck 8. Use of Reverse Polarity DNA to investigate Helicase MechanismsTim Lohman9. Substrate production to study helicases and translocases using molecular and optical tweezersFernando Moreno Herrero10. Methods of loading CMG onto DNAMichael O’Donnell11. Methods to study Helicase-Polymerase coupling during DNA replication reactionsSmita Patel12. Biochemical analyses of ATP-dependent chromatin remodelingCraig Peterson13. Alignment of helicases on single-stranded increases activityKevin D. Raney14. CMG helicase activity on G4-containing templatesDirk Remus15. Accessory helicase unwinding of genomic complexitiesGrant Schauer16. Monitoring helicase decoupling in bacteria by TUNELMichael Trakselis17. the preparation of DNA constructs for the use in single-molecule replisome studiesAntoine Van Oijen18. Interaction of topoisomerases with DNA measured by RADARAlessandro Vindigni