Enzymes as Sensors

Richard B. Thompson is Associate Professor at Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, U.S.A. Carol Fierke is Professor and Chair at the Department of Chemistry and Professor of Biological Chemistry at the Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, U.S.A.

• 1. Recent Advances in Development of Genetically Encoded Fluorescent Sensors2. Engineering Rugged Field Assays to Detect Hazardous Chemicals Using Spore-Based Bacterial Biosensors3. Engineering BRET-Sensor Proteins4. Enzymes as Sensors5. Integrated Strategies to Gain a Systems-Level View of Dynamic Signaling Networks6. Probing Cdc42 Polarization Dynamics in Budding Yeast Using a Biosensor7. Novel Fluorescence-Based Biosensors Incorporating Unnatural Amino Acids8. Folding- and Dynamics-Based Electrochemical DNA Sensors9. Construction of Protein-Based Biosensors Using Ligand-Directed Chemistry for Detecting Analyte Binding10. Measuring and Imaging Metal Ions With Fluorescence-Based Biosensors: Speciation, Selectivity, Kinetics, and Other Issues11. Bioapplications of Electrochemical Sensors and Biosensors12. A Highly Sensitive Biosensor for ATP Using a Chimeric Firefly Luciferase13. Highly Modular Bioluminescent Sensors for Small Molecules and Proteins14. Sensitive Protein Detection and Quantification in Paper-Based Microfluidics for the Point of Care15. Microneedle Enzyme Sensor Arrays for Continuous In Vivo Monitoring16. Visualization of the Genomic Loci That Are Bound by Specific Multiprotein Complexes by Bimolecular Fluorescence Complementation Analysis on Drosophila Polytene Chromosomes