ELISA and Other Solid Phase Immunoassays

Theoretical and Practical Aspects

Häftad, Engelska, 1988

AvKemeny,CHALLACOMB,D. M. Kemeny,S. J. Challacombe

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This is a quick-reference manual on practical aspects of immunoassay. Providing a theoretical and practical basis for modern applications of solid-phase immunoassays, this text brings together experts who have used ELISA and other assays in a variety of fields. Contributors offer step-by-step guidance on how to use the various techniques involved in immunoassay. These techniques are extemely useful to laboratory-based researchers and technicians working on the detection of allergy, the AIDS virus, autoimmunity, etc. Chapters analyze the solid-phase supports used, the amplification systems, and the quantitation and affinity of antibodies and discuss the applications of assays to biology, immunology, and microbiology.

Produktinformation

Utgivningsdatum1988-04-30
Mått158 x 233 x 23 mm
Vikt567 g
FormatHäftad
SpråkEngelska
Antal sidor384
FörlagJohn Wiley & Sons Inc
ISBN9780471909828

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Onkologi inom Medicin

BASIC ASPECTS. An Introduction to ELISA (D. Kemeny & S. Chanter). Microtitre Plates and Other Solid Phase Supports (D. Kemeny & S. Challacombe). Quantitative Aspects of Solid Phase Immunoassays (R. Hamilton & N. Atkinson). AMPLIFICATION. Amplification by Second Enzymes (A. Johannsson & D. Bates). The Amplified ELISA (a-ELISA): Immunochemistry and Applications (J. Butler). MOLECULAR ASPECTS. The Role of Antibody Affinity in the Performance of Solid Phase Assays (M. Devey & M. Steward). The Immunochemistry of Sandwich ELISAs: Principles and Applications for the Quantitative Determination of Immunoglobulins (J. Butler). The Use of ELISA in the Characterization of Protein Antigen Structure and Immune Response (A. Pesce & J. Michael). The Modified Sandwich ELISA (SELISA) for the Detection of IgE and Other Antibody Isotypes (D. Kemeny). CELLULAR ELISAs. The Solid Phase Enzyme-Linked Immunospot Assay (ELISPOT) for Enumerating Antibody-Secreting Cells: Methodology and Applications (C. Czerkinsky, et al.). ELISA-Plaque Assay for the Detection of Single Antibody-Secreting Cells (J. Sedgwick & P. Holt). CHEMILUMINESCENCE. Chemiluminescence Immunoassay (I. Weeks & J. Woodhead). APPLICATIONS TO MICROBIOLOGY. The Use of ELISA for Rapid Viral Diagnosis: Viral Antigen Detection in Clinical Specimens (S. Chantler & A. Clayton). The Use of ELISA for Rapid Viral Diagnosis: Antibody Detection (R. Duncan). Application of ELISA to Bacteriology (S. Challacombe). Appendix. Index.