Cardiovascular Development

• Use of Whole Embryo Culture for Studying Heart Development.-Quantifying Cardiac Functions in Embryonic and Adult Zebrafish.-Analysis of the Patterning of Cardiac Outflow Tract and Great Arteries with Angiography and Vascular Casting.-Morpholino Injection in Xenopus.-Chicken Chorioallantoic Membrane Angiogenesis Model.-Visualizing Vascular Networks in Zebrafish: An Introduction to Microangiography.-Whole-mount Confocal Microscopy for Vascular Branching Morphogenesis.-Visualization of Mouse Embryo Angiogenesis by Fluorescence-based Staining.-Miniaturized Assays of Angiogenesis in Vitro.-Analysis of the Endocardial-to-Mesenchymal Transformation of Heart Valve Development by a Collagen Gel Culture Assay.-Quantification of Myocyte Chemotaxis: A role for FAK in Regulating Directional Motility.-Analysis of Neural Crest Cell Fate During Cardiovascular Development using Cre-activated lacZ/b-galactosidase Staining.-Indirect Immunostaining on Mouse Embryonic Heart for the Detection of Proliferated Cardiomyocyte.-Isolation and Characterization of Vascular Endothelial Cells from Murine Heart and Lung.-Isolation and Characterization of Embryonic and Adult Epicardium and Epicardium-Derived Cells.-Vascular Smooth Muscle Cells:  Isolation, Culture, and Characterization.-C-kit Expression Identifies Cardiac Precursor Cells.-Cardiomyocyte Apoptosis in Heart Development: Methods and Protocols.-Adenovirus-mediated Gene Transfection in the Isolated Lymphatic Vessels.-Isolation of Cardiac Myocytes and Fibroblasts from Neonatal Rat Pups.-The Application of Genome-wide RNAi Screens in Exploring Varies of Signaling Transduction Pathways.-Application of Atomic Force Microscopy Measurements on Cardiovascular Cells.-In Utero Assessment of Cardiovascular Function in the Embryonic Mouse Heart using High Resolution Ultrasound Biomicroscopy.-Isolation and Preparation of RNA from Rat Blood and Lymphatic Microvessels for Use in Microarray Analysis.-Visual Data Mining of Co-expression Data to Set Research Priorities in Cardiac Development Research.-High-speed Confocal Imaging of Zebrafish Heart Development.-Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode Arrays.

From the reviews:“This is an up-to-date review of current research techniques used in the field of cardiovascular development. … The book successfully instructs researchers on proper experimental techniques, giving step-by-step instructions, required for research in this field. … this book is intended for use by both novice and experienced basic science researchers. … This book is an excellent reference for any researcher in this area.” (Patrick T. Campbell, Doody’s Book Reviews, April, 2012)