Del i serien Methods in Molecular Biology

Bacteriophages

Methods and Protocols, Volume 2: Molecular and Applied Aspects

Inbunden, Engelska, 2008

3 549 kr

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Ranging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community.

Produktinformation

Utgivningsdatum2008-12-15
Mått203 x 276 x 27 mm
Vikt1 163 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor373
Upplaga2009
FörlagHumana Press Inc.
ISBN9781603275644

Tillhör följande kategorier

Bacteriophage Genomics.- Preparation of Bacteriophage Lysates and Pure DNA.- Approaches to the Compositional Analysis of DNA.- Determination of Bacteriophage Genome Size by Pulsed-Field Gel Electrophoresis.- Preparation of a Phage DNA Fragment Library for Whole Genome Shotgun Sequencing.- PCR and Partial Sequencing of Bacteriophage Genomes.- In Sffamily Identification of Genes in Bacteriophage DNA.- Determining DNA Packaging Strategy by Analysis of the Termini of the Chromosomes in Tailed-Bacteriophage Virions.- In silico Characterization of DNA Motifs with Particular Reference to Promoters and Terminators.- Molecular Phylogenetics: Testing Evolutionary Hypotheses.- Bacteriophage Transcriptomics and Proteomics.- Preparation of RNA from Bacteria Infected with Bacteriophages: A Case Study from the Marine Unicellular Synechococcus sp. WH7803 Infected by Phage S-PM2.- Quantification of Host and Phage mRNA Expression During Infection Using Real-Time PCR.- Oligonucleotide Microarrays for Bacteriophage Expression Studies.- Purification of Bacteriophages and SDS-PAGE Analysis of Phage Structural Proteins from Ghost Particles.- Phage Proteomics: Applications of Mass Spectrometry.- Community Bacteriophage Approaches.- Isolation Independent Methods of Characterizing Phage Communities 1: Strain Typing Using Fingerprinting Methods.- Isolation Independent Methods of Characterizing Phage Communities 2: Characterizing a Metagenome.- Applied Aspects Of Bacteriophage Biology.- Phage Typing.- A Genetic Screen to Identify Bacteriophage Lysins.- General M13 Phage Display: M13 Phage Display in Identification and Characterization of Protein–Protein Interactions.- Isolation of Monoclonal Antibody Fragments from Phage Display Libraries.- Internet Resources of Interest to BacteriophageWorkers.