Animal Cell Culture

Inbunden, Engelska, 2024

AvRatan Kumar Choudhary,Manu M,Yashpal Singh Malik

2 699 kr

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Animal cell culture is a common laboratory technique for maintaining live cells separated from their original tissue (primary cells) or immortal cells (cell lines). The technique of cell culture was discovered in the 19th century after the discovery of the microscope.Presently, the culture of cells is one of the most important tools of life sciences research and is being used by investigators to understand advanced understanding of cell growth and differentiation and mechanisms of normal/ abnormal functioning of various cells. In addition to that, cell culture is employed in the generation of vaccines, the production of pharmaceutical proteins, and the safety testing of drugs.

Produktinformation

Utgivningsdatum2024-10-08
Mått108 x 229 x 0 mm
Vikt300 g
FormatInbunden
SpråkEngelska
SerieProtocol Series
Antal sidor108
FörlagNew India Publishing Agency
ISBN9789358879704

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Djurböcker inom Djur och Natur
Zoologi inom Naturvetenskap och teknik
Djurhållning inom Naturvetenskap och teknik

1 Layout, Safety Protocols and Guidelines in Cell Culture Facilities2 Basic Equipment of Animal Cell Culture Laboratories3 Washing, Packing, and Sterilization of Glass and Plastic Wares4 Preparation of Cell Culture Media and Reagents5 Isolation of Primary Culture of Chicken Embryo Fibroblast6 Isolation of Primary Culture of Canine Keratinocytes7 Isolation of Primary Culture of Canine Fibroblast8 Culture and Sub-culturing of Continuous Cell Lines9 Trypan Blue Dye Exclusion Cell Viability Assay10 Isolation and Culture Techniques for Lymphocytes11 Cryopreservation Methods of Primary Cultures and Cell Lines12 Evaluating Cytopathic Effects of Viruses on Mammalian Cells13 Virus Isolation in Cell Culture System14 One-Step Growth Curve of a Virus15 Production of Virus in Cell Culture16 Quantitative Analysis of Virus Titers through In-Vitro Assays17 Hemadsorption (HA) Assay for Virus Titration18 Plaque Assay for Virus Detection and Quantification19 Virus Neutralization Test20 Techniques for Establishing and Maintaining Cell Suspension Cultures21 Development of Cell lines Through Virus-Mediated Techniques22 Cell Viability and Cell Proliferation Assay