Advanced Microscopy

Takayoshi Kobayashi was born in Niigata Prefecture in 1944. He is a professor emeritus at the University of Tokyo, from which he previously graduated with Bachelor's, Master's, and Doctorate degrees. He joined the Institute of Physical and Chemical Research (Riken), and between 1977 and 1979, he was a temporary member of the Technical Staff at Bell Laboratories. In 1980 he joined the Department of Physics at the University of Tokyo as an associate professor and was promoted to full professor in 1994. In March 2006 he retired from the university and moved to the Department of Applied Physics and Chemistry at the University of Electro-Communications, in Tokyo. He was appointed Chair Professor and the Director of the National Chiao-tung University in 2006, which became the National Yang-Ming Chiao-Tung University in 2021. His research interests include quantum electronics, laser physics, femtosecond spectroscopy, ultrafast nonlinear optics, quantum optics, chemical physics, quantum information science and technology.

• Chapter 1 IntroductionChapter 2.1.1 Optimal Detection Angle in Sub-diffraction Resolution Photothermal Microscopy: Application for High-sensitivity Imaging of Biological TissuesChapter 2.1.2 Resolution Enhanced Pump-probe Microscope with a Spatial FilterChapter 2.1. Reduction of Distortion in Photothermal Microscopy and its Application to the High-resolution Three-dimensional Imaging of Nonfluorescent TissuesChapter 2.2.1 Numerical Study of the Subtraction Threshold for Fluorescence Difference MicroscopyChapter 2.2.2 Sub-Diffraction-Limit Imaging Using Mode MultiplexingChapter 2.2.3 Polarization Modulation for Fluorescence Emission Difference MicroscopyChapter 2.2.4 Numerical Study of Superresolved Optical Microscopy with Partly Staggered BeamsChapter 2.2.5 Resolution Enhancement of Pump-probe Microscopy with an Inverse-annular Spatial FilterChapter 3.1 Sub-Diffraction Resolution Pump-probe Microscopy with Shot-Noise Limited Sensitivity Using Laser DiodesChapter 3.2 Sensitivity Enhancement of Photothermal Microscopy with Radially Segmented Balanced DetectionChapter 3.3 Fast 3D Visualization of Endogenous Brain Signals with High-sensitivity Laser Scanning Photothermal MicroscopyChapter 5.2 Label-Free Imaging of Melanoma with Nonlinear Photothermal Microscopy3Chapter 5.3 Noninvasive, Label-free, Three-dimensional Imaging of Melanoma with Confocal Photothermal Microscopy: Differentiate Malignant Melanoma from Benign Tumor TissueChapter 5.4 Label-Free Imaging of Melanoma with Confocal Photothermal Microscopy: Differentiation between Malignant and Benign TissueChapter 6.1 Development of a Multiplex Stimulated Raman Microscope for Spectral Imaging through Multi-channel Lock-in DetectionChapter 6.2 Multiplex Stimulated Raman Imaging with White Probe-light from a Photonic-crystal Fiber and with Multi-wavelength Balanced DetectionChapter 6.3 Theoretical Description for Nonlinear Dynamic Light Scattering Based on Stimulated Raman Effect (NLDLS-SRS)