Advanced Microscopy

This book covers the principle, structure, enhancement of sensitivity and resolution power of photothermal and Raman microscopies. It includes real-world applications to biological and medical targets. Advanced Microscopy: Photo-Thermal and Induced-Raman Microscopy introduces clear descriptions of various Raman processes such as spontaneous, stimulates, coherent anti-Stokes Raman (CARS), Raman loss and Stokes Raman (gain). It covers pump-probe microscopies using actinic (pump) laser and sensing (probe) laser resulting in improvement due to intrinsic nonlinearity, which provides an advantage in the imaging of nonfluorescent targets. The author also provides solutions to noise and sensitivity problems which are two most important concerns in the microscopy applications. Finally, the book also draws direct comparisons of the advantages and drawbacks of a Raman microscopes in comparison with photothermal microscopes. The book will be useful to researchers and non-specialists in biomedical fields using optics and electronics relevant to (optical) microscopes. It will also be a helpful resource to graduate students in the fields of biology and medical research who are using photothermal microscopes in their research.