A Structure-Function Toolbox for Membrane Transporter and Channels

Prof. Dr. Christine Ziegler works at the Department of Structural Biology, Max Planck Institute of Biophysics, Germany

• 1. Lipid Nanodiscs as a Tool for High-Resolution Structure Determination of Membrane Proteins by Single-Particle Cryo-EM2. SSM-Based Electrophysiology for Transporter Research3. Saposin-Lipoprotein Scaffolds for Structure Determination of Membrane Transporters4. Single-Molecule Fluorescence Studies of Membrane Transporters Using Total Internal Reflection Microscopy5. The MEMbrane Protein Single ShoT Amplification Recipe: MemStar6. Efficient Screening and Optimization of Membrane Protein Production in Escherichia coli7. Pinning Down the Mechanism of Transport: Probing the Structure and Function of Transporters Using Cysteine Cross-Linking and Site-Specific Labeling8. Ion Channel Conformation and Oligomerization Assessment by Site-Directed Spin Labeling and Pulsed-EPR9. Strategy for the Thermostabilization of an Agonist-Bound GPCR Coupled to a G Protein

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