The Lymphatic Fluid and T-Cell Receptor Vβ expression in Peripheral Blood Mononuclear Cells (PBMCs) of patients with Wuchereria Bancrofti Infection

Doctoral Thesis / Dissertation from the year 2006 in the subject Medicine - Biomedical Engineering, grade: TEXTBOOK-PUBLICATION, Anna University (-), course: Ph.D.,, language: English, comment: A THESIS Submitted by M. SOLOMON SUDHAKAR in the fulfilment for the award of the degree of DOCTOR OF PHILOSOPHY , abstract: Lymphatic filariasis (LF) is a disease that is currently the target of a major global initiative for elimination.During the past decade, both the treatment and the control strategies for LF have undergone major paradigm shifts-due to rapid increase in knowledge and understanding of LF that is derived directly from a series of commendable progress made by scientific and medical research communities.In pursuit of understanding LF a disease to which a social stigma is attached, the chronic pathology (CP) of LF was taken as a model to understand the immunopathological mechanisms that lead to acute lymphangitis and lymphadenitis,in filarial infections, harboring Wuchereria bancrofti.It is a well established fact that elephantiasis is a consequence of immune-reactivity to adult worm antigens. Therefore,it was thought that T-cells infiltrating the lesions in chronic-pathology disease could augment for elevated inflammation seen in CP.An attempt was made to examine T cells by T Cell Receptor V analysis using parasite (BmA) and non parasite (PPD) antigen stimulated PBMC's for RT PCR and agarose gel electrophoresis followed by Integrated Density Values(IDV),analysis which were further validated by Statistical analysis using One-Way ANOVA followed by Tukeys-HSD Test.Therefore,primers designed for specific 24 V gene families in the given repertoire were the choice for experimental analysis by PCR. Five CP cases and 5 Endemic Normals who were normal healthy individuals' considered as controls,and also 2 Microfilaraemics, who are carriers of this disease were inducted into the current study. Specific T-Cell Receptors-TCRV1,TCRV2,TCRV7,TCRV14,TCRV20, TCRV24 wh