Techniques for Biochemical Analysis

Häftad, Engelska, 2024

AvKhursheed Hussain,Sameena Maqbool Lone,Khalid Z. Masoodi,Syed Minu Balkhi

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Techniques for Biochemical Analysis provides researchers with a practical guide for investigating a variety of different biomolecules. It includes a range of tried and tested protocols, outlining the principles upon which each technique is founded, as well as providing instructions on equipment setup and use, buffer preparation, reagents required, safety considerations and analysis of findings. Beginning with an introduction to biochemistry and laboratory procedures, the book moves on to specific methods focused on investigation of carbohydrates, proteins, enzymes, plant hormones, minerals, amino acids, and more.

The large range of protocols covered in this foundational, how-to reference are interdisciplinary and adaptable to a variety of areas, making this an ideal resource for researchers across various fields, including biochemistry, molecular biology, medical sciences, plant physiology, agriculture, and related subjects.

Features step-by-step methods for biochemical analysis of a variety of compounds
Explores methods that are applicable and adaptable across a variety of fields, including biochemistry, molecular biology, and related areas
Provides detailed instructions on how to prepare buffers, the equipment to be used, and the analysis of a variety of molecules, including carbohydrates, lipids, proteins, and hormones
Contains interdisciplinary and adaptable methods and techniques

Produktinformation

Utgivningsdatum2024-04-09
Mått191 x 235 x 16 mm
Vikt540 g
FormatHäftad
SpråkEngelska
Antal sidor256
FörlagElsevier Science
ISBN9780443159145

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Khursheed Hussain, has been serving as Assistant Professor at Sher-e-Kashmir University of Sciences & Technology in India for 13 years. He served as Scientific Officer to Vice Chancellor from 2015-2020. He has published more than 120 research articles, 25 review articles and many books. His contribution in the field of research is in varietal and hybrid development as Principal/Co-breeder of 7 vegetable varieties with other varieties in the pipeline. He has been an advisor of more than 50 PG students and has been invited to give many lectures and trainings. Sameena Maqbool Lone is a biotechnology researcher with a strong focus on vegetable science, molecular biotechnology, and sustainable bioproduction. She is currently a postdoctoral researcher in the Division of Vegetable Science, Sher-e- Kashmir University of Agricultural Sciences and Technology of Kashmir, India. Her current work explores the modulation of inflammatory and oncogenic signalling pathways, positioning plant-derived compounds within the broader framework of translational cancer therapeutics. Her research interests include phytochemical science, molecular diversity analysis, and the application of plant resources for health-related innovation. She holds a number of patents and has co-authored several research papers and books. Khalid Z. Masoodi is an Associate Professor and Senior Scientist in the Division of Plant Biotechnology at Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, India. He is the recipient of a number of awards and holds numerous patents. He has two decades worth of teaching and research experience in the areas of plant biotechnology, molecular biology, cancer research, and human disease research, and has published numerous articles and books. He leads a transcriptomics laboratory and works extensively with fluorescence imaging, real-time PCR, cell culture, and Western blotting platforms. His scientific interests include transcription biology in plant stress responses and cancer, as well as the discovery of new drugs from medicinal plants.Dr. Syed Minu Balkhi holds a PhD in Biochemistry from the University of Kashmir, India. She qualified for the JRF-NET CSIR and obtained a fellowship during her PhD program in 2013. She is currently a Lecturer at Government Boys Higher Secondary School, Humhama, India. Prior to this she was a Post-Doctoral Associate at the Department of Immunology, University of Pittsburgh, USA. She has contributed to various publications.

1. Introduction to2. Introduction to laboratory procedures3. Preparation of solutions and buffers4. Qualitative analysis of carbohydrates5. Qualitative analysis of proteins6. pH (negative logarithm of hydrogen ion concentration)7. Spectrophotometry8. Carbohydrates9. Amino acids and proteins10. Enzyme assays11. Vitamin assays12. Phenolics13. Plant pigments14. Plant15. Antinutritional factors in plants16. Toxins17. Extraction and identification of polyacetylenes18. Alkaloids19. Plant hormones20. Immunochemical methods21. Nitrogenase activity22. Chlorophyll23. Proximate analysis24. Minerals25. Antinutritional factors in plants26. Antioxidants27. Lipids