Scientific Principles of Adipose Stem Cells

Dr. Kokai is co-director of the Adipose Stem Cell Center at the University of Pittsburgh, faculty of the McGowan Institute of Regenerative Medicine (MIRM) and research faculty member in the Department of Plastic Surgery, School of Medicine. Dr. Kokai has a broad background in soft tissue engineering with specific training in development of novel biomaterials and drug delivery systems for adipose tissue regeneration and use of mesenchymal stem cells for tissue engineering and cell therapy applications. She has extensive experience in developing bioassays for biologic product development and has led collaborations with industry partners to transition preclinical research into commercialized products. Dr. Kokai is a co-Investigator in an NIH funded line of research aimed at developing cell-based methods for clinical soft tissue reconstruction after cancer therapy. Dr. Marra is an Associate Professor in the Department of Plastic Surgery (Primary), and Department of Bioengineering (Secondary), at the University of Pittsburgh. Dr. Marra’s academic training was in the areas of polymer chemistry, biomaterials and tissue engineering. She is the Director of the Plastic Surgery Research Laboratory (since 2002) and a core faculty member in the McGowan Institute for Regenerative Medicine, where she has served as a member of the Executive Committee since 2004. She has a publication record of 114 peer-reviewed publications, 24 reviews, 14 book chapters, and 4 patents, and over 570 abstracts in the areas of nerve regeneration, adipose stem cells, bioreactors, tissue engineering, and wound healing. Her funding includes NIH, NSF, and DoD grants in the area of regenerative medicine (including the Armed Forces Institute for Regenerative Medicine, (AFIRM)). She is the Enabling Technology Core Focus Area Leader in AFIRM II. She was Co-Chair of the 2015 Tissue Engineering and Regenerative Medicine International Society (TERMIS) World Congress, held in Boston. Dr. Rubin is endowed Professor of Plastic Surgery and Professor of Bioengineering, at the University of Pittsburgh with a faculty appointment with the McGowan Institute of Regenerative Medicine (MIRM). Dr. Rubin developed a basic science research program in the biology of adipose-derived stem cells and serves as Co-Director of the Adipose Stem Cell Center at the University of Pittsburgh. Dr. Rubin holds leadership positions in national and international plastic surgery societies, is the principal investigator in an NIH-funded line of research aimed at developing cell based methods for clinical soft tissue reconstruction after cancer therapy and holds two FDA cell therapy IDE protocols and an IND, all as sponsor/ investigator. Dr. Rubin’s many scientific leadership positions include past president of the International Society of Adipose Therapeutics and Science and past Chairman of the Plastic Surgery Research Council. He has received numerous awards for research, including the highly competitive Gingrass Award from the Plastic Surgery Research Council. He is the recipient of the American Association of Plastic Surgeons Academic Scholar Award, and the Plastic Surgery Educational Foundation Award for Outstanding Achievement in Basic and Translational Science. In 2007, Dr. Rubin was selected to receive a Presidential Early Career Award for Scientists and Engineers (PECASE). Dr. Rubin contributes expertise in scientific models assessing the use of adult adipose stem cell therapies for tissue engineering and regenerative applications

• Part 1 Introduction and front matter1. Plastic surgery, fat, and fat plasticity: How adipose tissue changed the landscape of stem cell therapeuticsLauren Kokai and J. Peter RubinPart 2 Basic biology of adipose stem cells2. Developmental origins of adipocytes: What we learn from human pluripotent stem cells Xi Yao, Vincent Dani, and Christian Dani3. Establishing the adipose stem cell identity: Characterization assays and functional propertiesMark A.A. Harrison, Sara I. Al-Ghadban, Benjamen T. O’Donnell, Omair A. Mohiuddin, Rachel M. Wise, Brianne N. Sullivan, and Bruce A. Bunnell4. Insights into the adipose stem cell niche in health and diseaseAaron C. Brown5. Mechanisms of adipose-derived stem cell aging and the impact on therapeutic potentialXiaoyin Shan and Ivona Percec6. Human pluripotent nontumorigenic multilineage differentiating stress enduring (Muse) cells isolated from adipose tissue: A new paradigm in regenerative medicine and cell therapyKaren L. Leung and Gregorio D. Chazenbalk7. Adipose stem cell homing and routes of delivery Ganesh Swaminathan, Yang Qiao, Bhavesh D. Kevadiya, Lucille A. Bresette, Daniel D. Liu, and Avnesh S. Thakor8. Bioreactors and microphysiological systems for adipose-based pharmacologic screeningMallory D. Griffin and Rosalyn D. AbbottPart 3  Adipose cell therapy and regenerative medicine9. Adipose stem cells and donor demographics: Impact of anatomic location, donor sex, race, BMI, and healthAdam Cottrill, Yasamin Samadi, and Kacey G. Marra10. Immunomodulatory properties of adipose stem cells in vivo: Preclinical and clinical applicationsMatthias Waldner, Fuat Baris Bengur, and Lauren Kokai11. Clinical experience with adipose tissue enriched with adipose stem cellsShawn Loder, Danielle Minteer, and J. Peter Rubin12. Adipose-derived stem cells for wound healing and fibrosisY Samadi, FM Egro, RL Rodriguez, and A Ejaz13. Oncologic safety of adipose-derived stem cell applicationHakan Orbay and David E. Sahar14. FDA regulation of adipose cell use in clinical trials and clinical translationMary Ann Chirba, Veronica Morgan Jones, Patsy Simon, and Adam J. KatzPart 4 Engineering with adipose stem cell15. Biomaterial control of adipose-derived stem/stromal cell differentiationJohn Walker and Lauren Flynn16. Genetic modification of adipose-derived stem cells for bone regenerationHarsh N. Shah, Abra H. Shen, Sandeep Adem, Ankit Salhotra, Michael T. Longaker, and Derrick C. Wan17. Adipose-derived stromal/stem cells for bone tissue engineering applicationsNathalie Faucheux, Fabien Kawecki, Jessica Jann, Franc¸ois A. Auger, Roberto D. Fanganiello, and Julie Fradette18. The hematopoietic potential of stem cells from the adipose tissueBeatrice Cousin and Louis Casteilla19. Adipose stem cells for peripheral nerve engineeringBenjamin K. Schilling, George E. Panagis, Jocelyn S. Baker, and Kacey G. Marra