Genetic engineering

This book covers the following topics: Recombinant DNA technology Restriction endonucleases T4 DNA ligase Plasmid cloning vectors pBR322 plasmid pUn9 plasmid Bacteriophage vectors Cosmids Transformation and selection Cloning DNA sequences that encode eukaryotic protein Synthesis of cDNA Creating and Screening a library Library construction methods Random mutagenesis methods Recombination-based methods Homology-dependent methods Homology-independent methods Targeted mutagenesis methods Selection/screening for desired property Genotype-phenotype linkage DNA sequencing techniques PCR Protein engineering Directed evolution Applications of directed evolution tool Rational design and site directed mutagenesis