Diabetes Mellitus

• Insulin Production.- Isolation of Islets of Langerhans from Rodent Pancreas.- Purification of Rat Pancreatic ?-Cells by Fluorescence-Activated Cell Sorting.- Assessment of Insulin Secretion in the Mouse.- Detection of Insulin Production by Immunohistochemistry.- Quantification of the Level of Insulin Gene Expression.- Chromatin Immunoprecipitation Using Isolated Islets of Langerhans.- Adenoviral Gene Transfer into ?-Cell Lines.- Utilization of NOD Mice in the Study of Type 1 Diabetes.- Insulin Action.- of DNA into 3T3-L1 Adipocytes by Electroporation.- Analysis of Insulin-Stimulated Glucose Uptake in Differentiated 3T3-L1 Adipocytes.- Fractionation Analysis of the Subcellular Distribution of GLUT-4 in 3T3-L1 Adipocytes.- Visualization and Quantitation of Integral Membrane Proteins Using a Plasma Membrane Sheet Assay.- Assaying Tyrosine Phosphorylation of Insulin Receptor and Insulin Receptor Substrates.- Measuring Insulin-Stimulated Phosphatidyl-Inositol 3-Kinase Activity.- Assaying AKT/Protein Kinase B Activity.- Measurements of Cellular Phosphoinositide Levels in 3T3-L1 Adipocytes.- Measurement of Glycogen Synthesis and Glycogen Synthase Activity in 3T3-L1 Adipocytes.- Measurement of Contraction-Stimulated GLUT-4 Translocation in Isolated Skeletal Muscle.- Single Embryo Measurement of Basal-and Insulin-Stimulated Glucose Uptake.- Immunohistologic Staining of Muscle and Embryos to Detect Insulin-Stimulated Translocation of Glucose Transporters.

"This book provides all the critical tools needed for the productive study of diabetes mellitus today." - Clinical Laboratory International "Each chapter/protocol is well referenced and the book is indexed. Because Diabetes Mellitus: Methods and Protocols provides all the critical tools needed for the productive study of diabetes mellitus, it is a necessary purchase for libraries supporting basic research programs that study the molecular and biochemical bases of diabetes mellitus." -E-STREAMS