Aminopeptidase from Marine Aspergillus Flavus

Amino peptidases have many important commercial applications. The food industry uses them for debittesing and improving the functional properties of protein products and the flavor development in cheese. In pharmaceutical industry inhibitors developing against this enzyme find application as drug. Amino peptidase is also used for peptide sequencing and in processing of recombinant proteins. The different species of fungi were isolated from marine soil sample and the Aspergillus flavus was dominantly present. The amino peptidase activity measured at different pH and temperatures was found to be maximal at pH 7 and temperature 45C. The maximum amino peptidase activity was found to be produced with the use of maltose and potassium nitrite. The molecular weight of the enzyme was determined by SDS-PAGE was found to be 120 Kilodaldon.