Acid resistant colliforms

Bacteria are those organisms which are single cell and they are not seen through naked eye they are only seen when microscope is used. The shape and size of bacteria are different and are measured in micrometer. Healthy humans are the reservoir and source of production of Shiga toxin producing Escherichia coli. The objective of this work is to define more precisely the role of traditional food ingredients in promoting the growth of acid resistant Coli form bacteria in human beings. Different food samples of Yogurt, Dahi bhallay and Fruit chart were taken in measured quantity in sterilized tubes and were soon be transported to lab. Different dilutions were prepared in sterile water. Initially the material was homogenized in measured amount of sterile water. The diluted portion of the suspensions of the samples was exposed to different concentration of Hydro caloric acid for different periods of time. Then the aliquots were spread over EMB agar plates which were subsequently incubated at 37 degree centigrade. Then colonies of Coliform bacteria were counted and acid resistance of the exposed bacteria was further worked out.