A clinical, bacteriological & mycological profile among pt's with csom

The present study was conducted at Department of Microbiology of Vydehi Institute Of Medical Science and Research Centre, Bangalore on 100 clinically diagnosed CSOM patients attending ENT OPD were studied for bacteriological (aerobic and anaerobic) and fungal flora from January 2017 to June 2018.Ear discharge was collected under strict aseptic precautions from the affected ear of CSOM cases. Excess discharge was mopped up from external auditory canal and it was cleaned with 70% alcohol first and was allowed to dry for 30-40 seconds to achieve sterile area. Then with four sterile swabs specimen was collected. 1st swab was for gram staining and wet mount preparation using 10%.KOH . 2nd swab was subjected for aerobic culture on Blood agar, MacConkey agar and Chocolate agar. 3rd one was used for anaerobic culture by inoculated in Robertson Cooked Meat media soon after the collection of the specimen. 4th swab was used for mycological culture on two Sabouraud's dextrose agar plate without antibiotics. After isolation, Gram stain, biochemical reactions were done for bacterial isolates and LPCB mount , slide culture were done for fungal isolates according to the standard procedures.